Rabbit anti-Human RELA / NFKB p65 Polyclonal Antibody | anti-RELA antibody

Rabbit Polyclonal to Human RELA / NFKB p65

Cat. Num. AAA240395

• Gene Names: RELA; p65; NFKB3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: RELA / NFKB p65; Polyclonal Antibody; Rabbit Polyclonal to Human RELA / NFKB p65; Anti-RELA / NFKB p65 Antibody IHC-plus; RELA; NF-kappa-B p65delta3; NFKB3; p65; Transcription factor p65; Human RELA; NFKB p65; anti-RELA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Residues surrounding S536 of human p65 (RelA) protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 551

• Applicable Applications for anti-RELA antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:1000 - 1:6000), IHC-P (5 ug/ml), WB (1:200 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human RELA.
• Immunogen Type: Synthetic peptide
• Immunogen: RELA / NFKB p65 antibody was raised against synthetic peptide from human RELA.
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-p65 (RelA) pS536 Antibody - Western Blot. Western blot of affinity purified anti-p65 (RelA) pS536 antibody shows detection of p65 phosphorylated at S536. The control blot (left lane) contains 100 ng of purified p65-GST fusion protein. The band is seen (right lane) when this protein is phosphorylated at S536 by IKKbeta. Personal Communication. J. Brady, NCI, Bethesda, MD.)

Related Product Information for anti-RELA antibody

NFKB1 or NFKB2 bind to REL, RELA, or RELB to form the NFKB complex. The NFKB1/RELA heterodimer is the most abundant form of NFKB. The NFKB complex is inactivated by I-kappa-B proteins that keep it trapped in the cytoplasm. Phosphorylation of serine residues on the I-kappa B proteins mark them for ubiquitination and destruction, and NFKB translocates to the nucleus where it binds DNA.

NCBI and Uniprot Product Information

• NCBI GI #: 223468676
• NCBI GeneID: 5970
• NCBI Accession #: NP_068810.3
• NCBI GenBank Nucleotide #: NM_021975.3
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: 59,910 Da
• NCBI Official Full Name: transcription factor p65 isoform 1
• NCBI Official Synonym Full Names: v-rel avian reticuloendotheliosis viral oncogene homolog A
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
• UniProt Protein Name: Transcription factor p65
• Protein Family: Transcription factor
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; NF-kappaB binding; identical protein binding; protein binding; DNA binding; transcription activator binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; protein N-terminus binding; chromatin binding; phosphate binding; transcription factor activity; transcription factor binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; response to cAMP; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of transcription, DNA-dependent; response to morphine; negative regulation of insulin receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; response to insulin stimulus; toll-like receptor 5 signaling pathway; response to organic substance; hair follicle development; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; defense response to virus; toll-like receptor 4 signaling pathway; aging; response to drug; positive regulation of Schwann cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; membrane protein intracellular domain proteolysis; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; acetaldehyde metabolic process; liver development; response to amino acid stimulus; toll-like receptor 2 signaling pathway; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; positive regulation of interleukin-12 biosynthetic process; response to mechanical stimulus; response to muramyl dipeptide; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; cellular defense response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to progesterone stimulus; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; response to cobalamin; negative regulation of apoptosis