Rabbit SUMO1 / SMT3 Polyclonal Antibody | anti-SUMO1 antibody

Rabbit Polyclonal to Human SUMO1 / SMT3

Cat. Num. AAA240324

• Gene Names: SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: SUMO1 / SMT3; Polyclonal Antibody; Rabbit Polyclonal to Human SUMO1 / SMT3; Anti-SUMO1 / SMT3 Antibody (N-Terminus) IHC-plus; SUMO1; GAP modifying protein 1; GMP1; OFC10; Sentrin; SMT3 homolog 3; SMT3C; SMT3H3; SUMO-1; Ubiquitin-like protein UBL1; PIC1; Ubiquitin-like 1 (sentrin); UBL1; DAP1; GAP-modifying protein 1; Ubiquitin-like protein SMT3C; Human SUMO1; SMT3; anti-SUMO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: 14 amino acid peptide from near the amino terminus of human sumo
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 101

• Applicable Applications for anti-SUMO1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human SUMO1.
• Immunogen Type: Synthetic peptide
• Immunogen: SUMO1 / SMT3 antibody was raised against synthetic peptide from human SUMO1.
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-SUMO1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SUMO1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of Sumo in HL60 cells with Sumo antibody at 20 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of Sumo in HL60 cells with Sumo antibody at 5 ug/ml.)

Western Blot (WB)

(Western blot of sumo in HL-60 cell lysate with sumo antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.)

Related Product Information for anti-SUMO1 antibody

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability.

NCBI and Uniprot Product Information

• NCBI GI #: 4507801
• NCBI GeneID: 7341
• NCBI Accession #: NP_003343.1
• NCBI GenBank Nucleotide #: NM_003352.4
• UniProt Accession #: P63165; P55856; Q6FGG0; Q6NZ62; Q93068; A8MUS8; B2R4I5
• Molecular Weight: 8,799 Da
• NCBI Official Full Name: small ubiquitin-related modifier 1 isoform a
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 1
• NCBI Official Symbol: SUMO1
• NCBI Official Synonym Symbols: DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
• NCBI Protein Information: small ubiquitin-related modifier 1; sentrin; SMT3 homolog 3; GAP modifying protein 1; ubiquitin-like protein UBL1; ubiquitin-like protein SMT3C; SMT3 suppressor of mif two 3 homolog 1; ubiquitin-homology domain protein PIC1
• UniProt Protein Name: Small ubiquitin-related modifier 1
• Protein Family: Small ubiquitin-related modifier
• UniProt Gene Name: SUMO1
• UniProt Synonym Gene Names: SMT3C; SMT3H3; UBL1; SUMO-1; GMP1; Smt3C
• UniProt Entry Name: SUMO1_HUMAN

NCBI Description

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

SUMO1: Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Interacts with SAE2, UBE2I, RANBP2, PIAS1 and PIAS2. Interacts with PARK2. Covalently attached to a number of proteins such as IKFZ1, PML, RANGAP1, HIPK2, SP100, p53, p73-alpha, MDM2, JUN, DNMT3B and TDG. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, EXOSC9, RAD51 and RAD52. Interacts with USP25 (via ts SIM domain); the interaction weakly sumoylates USP25. Belongs to the ubiquitin family. SUMO subfamily.

Protein type: Ubiquitin-like modifier; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: nucleoplasm; PML body; nuclear membrane; cytoplasm; dendrite; nucleolus; fibrillar center; nuclear pore; synapse; nuclear speck; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding; SUMO ligase activity; transcription factor binding

Biological Process: cellular protein metabolic process; regulation of protein localization; positive regulation of protein complex assembly; protein sumoylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; cytokine and chemokine mediated signaling pathway; negative regulation of transcription factor activity; PML body organization and biogenesis; palate development; DNA repair; negative regulation of transcription, DNA-dependent; post-translational protein modification; negative regulation of DNA binding

Disease: Orofacial Cleft 10