Mouse anti-Human NUMA1/NUMA Monoclonal Antibody | anti-NUMA1/NUMA antibody

Anti-NUMA1/NUMA Antibody (clone 1C5)

Cat. Num. AAA2401129

• Gene Names: NUMA1; NUMA; NMP-22
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified from ascites by Protein A
• Synonyms: NUMA1/NUMA; Monoclonal Antibody; Anti-NUMA1/NUMA Antibody (clone 1C5); Mouse Monoclonal [clone A73-B/D12] (IgM) to Mouse NUMA1/NUMA; NUMA1; Structural nuclear protein; NuMA protein; SP-H antigen; NUMA; anti-NUMA1/NUMA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 1C5
• Specificity: Human NUMA1/NUMA
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Sequence Length: 2115

• Applicable Applications for anti-NUMA1/NUMA antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IHC-P: 10 ug/ml
• Immunogen: NUMA1 (NP_006176, aa200-306 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C or lower.; Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(NUMA1 monoclonal antibody (M01), clone 1C5 Western Blot analysis of NUMA1 expression in K-562.)

Immunoprecipitation (IP)

(Immunoprecipitation of NUMA1 transfected lysate using anti-NUMA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NUMA1 rabbit polyclonal antibody.)

ELISA (EIA)

(Detection limit for recombinant GST tagged NUMA1 is approximately 0.1 ng/ml as a capture antibody.)

Related Product Information for anti-NUMA1/NUMA antibody

Highly abundant component of the nuclear matrix where it may serve anon-mitotic structural role, occupies the majority if the nuclearvolume. Required for maintenance and establishment of the mitoticspindle poles, functionning as a tether linking bulk microtubules of the spindle to centrosomes. May be involved in coordination of the alignment of the mitotic spindle to the cellular polarity axis, which isa prerequisite for asymmetric cell divisions.

NCBI and Uniprot Product Information

• NCBI GI #: 71361682
• NCBI GeneID: 4926
• NCBI Accession #: NP_006176.2
• NCBI GenBank Nucleotide #: NM_006185.3
• UniProt Accession #: Q14980; Q14981; Q9BTE9; H0YH75
• Molecular Weight: 109,279 Da
• NCBI Official Full Name: nuclear mitotic apparatus protein 1 isoform 1
• NCBI Official Synonym Full Names: nuclear mitotic apparatus protein 1
• NCBI Official Symbol: NUMA1
• NCBI Official Synonym Symbols: NUMA; NMP-22
• NCBI Protein Information: nuclear mitotic apparatus protein 1
• UniProt Protein Name: Nuclear mitotic apparatus protein 1
• UniProt Gene Name: NUMA1
• UniProt Synonym Gene Names: NuMA protein

NCBI Description

This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

Uniprot Description

Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed:7769006, PubMed:17172455, PubMed:19255246, PubMed:24996901, PubMed:26195665, PubMed:27462074). Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed:12445386, PubMed:11956313). Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed:23870127, PubMed:24109598, PubMed:24996901, PubMed:26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PubMed:23027904, PubMed:22327364, PubMed:23921553). During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation (PubMed:22327364, PubMed:23921553, PubMed:24996901, PubMed:24371089). Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (PubMed:24996901, PubMed:24371089). Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PubMed:21816348). Plays a role in mitotic MT aster assembly (PubMed:11163243, PubMed:11229403, PubMed:12445386). Involved in anastral spindle assembly (PubMed:25657325). Positively regulates TNKS protein localization to spindle poles in mitosis (PubMed:16076287). Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (PubMed:10075938). Required for epidermal differentiation and hair follicle morphogenesis ().