Rabbit SLC6A1 / GAT-1 Polyclonal Antibody | anti-SLC6A1 / GAT-1 antibody

Anti-SLC6A1 / GAT-1 Antibody (aa372-421) IHC-plus

Cat. Num. AAA2400172

• Gene Names: SLC6A1; MAE; GAT1; GABATR; GABATHG
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity purified
• Synonyms: SLC6A1 / GAT-1; Polyclonal Antibody; Anti-SLC6A1 / GAT-1 Antibody (aa372-421) IHC-plus; Rabbit Polyclonal (IgG) to Human SLC6A1 / GAT-1; Human SLC6A1 / GAT-1; GABA transporter; GABA transporter 1; GABT1; GAT1; GABATHG; GABATR; GAT 1; anti-SLC6A1 / GAT-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SLC6A1 antibody detects endogenous levels of SLC6A1.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-SLC6A1 / GAT-1 antibody: Immunohistochemistry - Paraffin (IHC - Paraffin), Western Blot (WB), Peptide Enzyme-Linked Immunosorbent Assay (Peptide ELISA)
• Application Notes: IHC - Paraffin (1:400); Western blot (1:500-1:1000); Peptide Enzyme-Linked Immunosorbent Assay (1:10000); (optimal dilution to be determined by the researcher)
• Host Note: SLC6A1 / GAT-1 antibody was produced in Rabbit.
• Immunogen Species: SLC6A1 / GAT-1 antibody was raised against Human.
• Immunogen: SLC6A1 / GAT-1 antibody was raised against synthetic peptide from human SLC6A1. (AA Range: 170-220)
• Epitope: aa372-421
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C.

Immunohistochemistry - Paraffin (IHC)

(Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot of the lysates from HeLa cells using SLC6A1 antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 188528618
• NCBI GeneID: 6529
• NCBI Accession #: NP_003033.3
• NCBI GenBank Nucleotide #: NM_003042.3
• UniProt Accession #: P30531; Q8N4K8
• Molecular Weight: Western Blot: Predicted MW 67kDa
• NCBI Official Full Name: sodium- and chloride-dependent GABA transporter 1
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 1
• NCBI Official Symbol: SLC6A1
• NCBI Official Synonym Symbols: MAE; GAT1; GABATR; GABATHG
• NCBI Protein Information: sodium- and chloride-dependent GABA transporter 1
• UniProt Protein Name: Sodium- and chloride-dependent GABA transporter 1
• UniProt Gene Name: SLC6A1
• UniProt Synonym Gene Names: GABATR; GABT1; GAT1; GAT-1
• UniProt Entry Name: SC6A1_HUMAN

NCBI Description

The SLC6A1 gene encodes a gamma-aminobutyric acid (GABA) transporter, which removes GABA from the synaptic cleft (Hirunsatit et al., 2009 [PubMed 19077666]).[supplied by OMIM, Jul 2009]

Uniprot Description

SLC6A1: Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily.

Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: axon; cell surface; integral to membrane; integral to plasma membrane; membrane; neuron projection; plasma membrane

Molecular Function: gamma-aminobutyric acid:sodium symporter activity; neurotransmitter:sodium symporter activity

Biological Process: gamma-aminobutyric acid import; gamma-aminobutyric acid transport; learning; negative regulation of synaptic transmission, GABAergic; neurotransmitter secretion; neurotransmitter transport; positive regulation of gamma-aminobutyric acid secretion; protein homooligomerization; response to calcium ion; response to cocaine; response to estradiol stimulus; response to lead ion; response to purine; response to sucrose stimulus; response to toxin; synaptic transmission; transmembrane transport; transport

Disease: Myoclonic-atonic Epilepsy