WB (Western Blot)
(Detection of human AXL by western blot. Samples: Whole cell lysate (10 ug) from OVCAR-8, HEK293T, HeLa, Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes.)
AXL recombinant antibody
Rabbit anti-AXL Recombinant Monoclonal Antibody [BLR222K]
IP: 6 ul/mg lysate
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human AXL by western blot. Samples: Whole cell lysate (10 ug) from OVCAR-8, HEK293T, HeLa, Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human AXL by western blot. Samples: Whole cell lysate (10 ug) from OVCAR-8, HEK293T, HeLa, Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse AXL by western blot. Samples: Whole cell lysate (25 ug) from AML12, C2C12, CT26, TCMK-1, and 4T1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse AXL by western blot. Samples: Whole cell lysate (25 ug) from AML12, C2C12, CT26, TCMK-1, and 4T1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human AXL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873 lot 1) used for IP at 6 ul/mg lysate. AXL was also immunoprecipitated by an antibody against a different epitope of AXL and a polyclonal antibody against the same epitope of AXL . For blotting immunoprecipitated AXL, AAA23873 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human AXL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873 lot 1) used for IP at 6 ul/mg lysate. AXL was also immunoprecipitated by an antibody against a different epitope of AXL and a polyclonal antibody against the same epitope of AXL . For blotting immunoprecipitated AXL, AAA23873 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
ICC (Immunocytochemistry)
(Detection of mouse AXL by immunocytochemistry. Sample: FFPE section of AML12 cells. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of mouse AXL by immunocytochemistry. Sample: FFPE section of AML12 cells. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human AXL by immunocytochemistry. Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human AXL by immunocytochemistry. Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human AXL by immunocytochemistry. Sample: FFPE section of DU145 cells. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human AXL by immunocytochemistry. Sample: FFPE section of DU145 cells. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human AXL (shaded) in OVCAR8 cells by flow cytometry. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human AXL (shaded) in OVCAR8 cells by flow cytometry. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
NCBI and Uniprot Product Information
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Product Notes
The AXL axl (Catalog #AAA23873) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-AXL Recombinant Monoclonal Antibody [BLR222K] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AXL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 6 ul/mg lysate ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the AXL axl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AXL, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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