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WB (Western Blot)
(Detection of human PU.1 by western blot. Samples: Whole cell lysate (50 ug) from THP-1, Jurkat, K-562, HEK293T, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
PU.1 recombinant antibody
Rabbit anti-PU.1 Recombinant Monoclonal Antibody [BLR178J]
Gene Names
SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
PU.1; Monoclonal Recombinant Antibody; Rabbit anti-PU.1 Recombinant Monoclonal Antibody [BLR178J]; SFPI1; transcription factor PU.1; spleen focus forming virus (SFFV) proviral integration oncogene spi1; 31 kDa transforming protein; SPI-1; OF; hematopoietic transcription factor PU.1; 31 kDa-transforming protein; SPI-A; Transcription factor PU.1; Spi-1 proto-oncogene; PU.1 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR178J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
500ug/ml (varies by lot)
Applicable Applications for PU.1 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 12 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
IP: 12 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PU.1 by western blot. Samples: Whole cell lysate (50 ug) from THP-1, Jurkat, K-562, HEK293T, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human PU.1 by western blot. Samples: Whole cell lysate (50 ug) from THP-1, Jurkat, K-562, HEK293T, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human PU.1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from THP-1 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853 lot 1) used for IP at 12 ul/mg lysate. PU.1 was also immunoprecipitated by a second antibody against a different epitope of PU.1 . For blotting immunoprecipitated PU.1, AAA23853 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human PU.1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from THP-1 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853 lot 1) used for IP at 12 ul/mg lysate. PU.1 was also immunoprecipitated by a second antibody against a different epitope of PU.1 . For blotting immunoprecipitated PU.1, AAA23853 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human PU.1 in tonsil by IHC. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
IHC (Immunohistochemistry)
(Detection of human PU.1 in tonsil by IHC. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
ICC (Immunocytochemistry)
(Detection of human PU.1 in THP-1 cells by ICC. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
ICC (Immunocytochemistry)
(Detection of human PU.1 in THP-1 cells by ICC. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
ICC (Immunocytochemistry)
(Detection of human PU.1 in HL-60 cells by ICC. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
ICC (Immunocytochemistry)
(Detection of human PU.1 in HL-60 cells by ICC. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
FCM (Flow Cytometry)
(Detection of human PU.1 (shaded) in K562 cells by flow cytometry. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human PU.1 (shaded) in K562 cells by flow cytometry. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23853) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for PU.1 recombinant antibody
PU.1 is an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. PU.1 binds to a purine-rich sequence known as the PU-box found near the promoters of target genes and regulates their expression in coordination with other transcription factors and cofactors. [taken from NCBI Entrez Gene (Gene ID: 6688)].
Product Categories/Family for PU.1 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,211 Da
NCBI Official Full Name
transcription factor PU.1 isoform 2
NCBI Official Synonym Full Names
Spi-1 proto-oncogene
NCBI Official Symbol
SPI1
NCBI Official Synonym Symbols
OF; PU.1; SFPI1; SPI-1; SPI-A
NCBI Protein Information
transcription factor PU.1; 31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1; spleen focus forming virus (SFFV) proviral integration oncogene spi1
UniProt Protein Name
Transcription factor PU.1
UniProt Gene Name
SPI1
UniProt Entry Name
SPI1_HUMAN
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Product Notes
The PU.1 spi1 (Catalog #AAA23853) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PU.1 Recombinant Monoclonal Antibody [BLR178J] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PU.1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 12 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the PU.1 spi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PU.1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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