Mouse Brain Natriuretic Peptide (BNP) ELISA Kit | BNP elisa kit

Mouse Brain Natriuretic Peptide (BNP) ELISA Kit

• Gene Names: NPPB; BNP
• Reactivity: Mouse
• Synonyms: Brain Natriuretic Peptide (BNP); Mouse Brain Natriuretic Peptide (BNP) ELISA Kit; GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32; BNP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of BNP. No significant cross-reactivity or interference between BNP and analogues was observed.
• Sequence Length: 1

• Assay Type: Competitive
• Samples: Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 12.35-1,000pg/mL
• Sensitivity: <4.81pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BNP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BNP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for BNP elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to BNP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled BNP and unlabeled BNP (Standards or samples) with the pre-coated antibody specific to BNP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of BNP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of BNP in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 4433127
• NCBI GeneID: 4879
• Molecular Weight: 14,726 Da
• NCBI Official Full Name: brain natriuretic peptide, partial
• NCBI Official Synonym Full Names: natriuretic peptide B
• NCBI Official Symbol: NPPB
• NCBI Official Synonym Symbols: BNP
• NCBI Protein Information: natriuretic peptides B
• UniProt Protein Name: Natriuretic peptides B
• UniProt Gene Name: NPPB
• UniProt Synonym Gene Names: BNP(1-32); BNP-32