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ELISA (EIA) (Sandwich ELISA analysis of human IL-32 using Mouse anti Human interleukin-32 as a capture reagent and biotinylated Mouse anti Human interleukin-32 (MBS226187) as a detection reagent with recombinant human IL-32 as the antigen. Detection was per)

Mouse anti-Human Interleukin-32 Monoclonal Antibody | anti-IL-32 antibody

Mouse anti Human Interleukin-32:Biotin

Gene Names
IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
Reactivity
Human
Applications
ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
Interleukin-32; Monoclonal Antibody; Mouse anti Human Interleukin-32:Biotin; IL-32 antibody | B01-2A7; anti-IL-32 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
B01-2A7
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Applicable Applications for anti-IL-32 antibody
ELISA (EIA)
Application Notes
ELISA: 1.0ug/ml
Immunogen
E Coli derived recombinant human interleukin-32 (Cys2-Lys131)
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Conjugation
Biotin
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA (EIA)

(Sandwich ELISA analysis of human IL-32 using Mouse anti Human interleukin-32 as a capture reagent and biotinylated Mouse anti Human interleukin-32 (MBS226187) as a detection reagent with recombinant human IL-32 as the antigen. Detection was per)

ELISA (EIA) (Sandwich ELISA analysis of human IL-32 using Mouse anti Human interleukin-32 as a capture reagent and biotinylated Mouse anti Human interleukin-32 (MBS226187) as a detection reagent with recombinant human IL-32 as the antigen. Detection was per)
Related Product Information for anti-IL-32 antibody
Mouse anti Human interleukin-32 antibody, clone B01-2A7, recognizes interleukin-32 (IL-32), also known as NK4 and TAIF. IL-32 is a pro-inflammatory cytokine with roles in inflammation and immune regulation, and its expression is strongly regulated by pathogens and pro-inflammatory cytokines (Hong et al. 2017). IL-32 promotes inflammation by upregulating other inflammatory cytokines including TNF-alpha, IL-1beta, IL-6, INF-gamma, and IL-8 (Al-Shobaili et al. 2018). Accordingly, a role for IL-32 has been reported in the pathogenesis of chronic inflammatory diseases including IBD, COPD and RA (Damen et al. 2017). IL-32 is highly expressed in several cancer tissues, and plays a crucial role in cancer development through tumor initiation, cell proliferation, and tumor maintenance (Yan et al. 2018).

The biotinylated Mouse anti Human interleukin-32 antibody, clone B01-2A7 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human interleukin-32 antibody, clone E03-4C9 as the capture antibody.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,676 Da
NCBI Official Full Name
interleukin-32 isoform B
NCBI Official Synonym Full Names
interleukin 32
NCBI Official Symbol
IL32
NCBI Official Synonym Symbols
NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
NCBI Protein Information
interleukin-32; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cells protein 4; natural killer cell transcript 4; tumor necrosis factor alpha-inducing factor
UniProt Protein Name
Interleukin-32
Protein Family
UniProt Gene Name
IL32
UniProt Synonym Gene Names
NK4; TAIF; IL-32
UniProt Entry Name
IL32_HUMAN

NCBI Description

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

IL32: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF- alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular space; membrane

Molecular Function: protein binding; cytokine activity

Biological Process: defense response; immune response; cell adhesion

Research Articles on IL-32

Similar Products

Product Notes

The IL-32 il32 (Catalog #AAA226187) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human Interleukin-32:Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin-32 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1.0ug/ml. Researchers should empirically determine the suitability of the IL-32 il32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin-32, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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