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ELISA (EIA) (Sandwich ELISA analysis of human IL-17F using Mouse anti Human interleukin-17F (MBS226173) as a capture reagent and biotinylated Mouse anti Human interleukin-17F as a detection reagent with recombinant human IL-17F as the antigen. Detection was)

Mouse anti-Human Interleukin-17F Monoclonal Antibody | anti-IL-17F antibody

Mouse anti Human Interleukin-17F

Gene Names
IL17F; ML1; ML-1; CANDF6; IL-17F
Reactivity
Human
Applications
ELISA
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
Interleukin-17F; Monoclonal Antibody; Mouse anti Human Interleukin-17F; IL-17F antibody | I08-5H8; anti-IL-17F antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
I08-5H8
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Phosphate buffered saline
0.09% Sodium Azide (NaN3)
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-IL-17F antibody
ELISA (EIA)
Application Notes
ELISA: 1.0ug/ml
Immunogen
E Coli derived recombinant human full length interleukin-17F (Arg31-Gln163)
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA (EIA)

(Sandwich ELISA analysis of human IL-17F using Mouse anti Human interleukin-17F (MBS226173) as a capture reagent and biotinylated Mouse anti Human interleukin-17F as a detection reagent with recombinant human IL-17F as the antigen. Detection was)

ELISA (EIA) (Sandwich ELISA analysis of human IL-17F using Mouse anti Human interleukin-17F (MBS226173) as a capture reagent and biotinylated Mouse anti Human interleukin-17F as a detection reagent with recombinant human IL-17F as the antigen. Detection was)
Related Product Information for anti-IL-17F antibody
Mouse anti Human interleukin-17F antibody, clone I08-5H8, recognizes interleukin-17 F, also known as cytokine ML-1. The IL-17 family consists of six proteins (IL-17A to IL-17F) which function as proinflammatory cytokines, important for defense against pathogens (Gurczynski and Moore 2017).

The purified Mouse anti Human interleukin-17F antibody, clone I08-5H8 can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human interleukin-17F antibody, clone H02-4D7 as the detection antibody.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,045 Da
NCBI Official Full Name
interleukin-17F
NCBI Official Synonym Full Names
interleukin 17F
NCBI Official Symbol
IL17F
NCBI Official Synonym Symbols
ML1; ML-1; CANDF6; IL-17F
NCBI Protein Information
interleukin-17F; cytokine ML-1
UniProt Protein Name
Interleukin-17F
Protein Family
UniProt Gene Name
IL17F
UniProt Synonym Gene Names
IL-17F
UniProt Entry Name
IL17F_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008]

Uniprot Description

IL17F: Stimulates the production of other cytokines such as IL- 6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6). CANDF6 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. Belongs to the IL-17 family.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: extracellular space; extracellular region

Molecular Function: protein homodimerization activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine binding; cytokine activity

Biological Process: proteoglycan metabolic process; negative regulation of angiogenesis; regulation of transforming growth factor beta receptor signaling pathway; regulation of interleukin-8 biosynthetic process; lymphotoxin A biosynthetic process; cartilage development; cytokine biosynthetic process; regulation of interleukin-2 biosynthetic process; regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; regulation of interleukin-6 biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; inflammatory response

Disease: Candidiasis, Familial, 6

Research Articles on IL-17F

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Product Notes

The IL-17F il17f (Catalog #AAA226173) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human Interleukin-17F reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin-17F can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1.0ug/ml. Researchers should empirically determine the suitability of the IL-17F il17f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin-17F, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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