Western Blot (WB)
(Western blot analysis of whole cell lysates probed with spectrin alpha chain antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 53 second exposure. Arrow points to spectrin alpha chain (molecular weight 285 kDa))
Mouse anti-Human Spectrin Alpha Chain Monoclonal Antibody | anti-SPTAN1 antibody
Spectrin Alpha Chain Antibody with Control Lysate
Western Blotting: Detects a band of approximately 285 kDa in Jurkat cell lysates.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with spectrin alpha chain antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 53 second exposure. Arrow points to spectrin alpha chain (molecular weight 285 kDa))
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with spectrin alpha chain antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc MP with 53 second exposure. Arrow points to spectrin alpha chain (molecular weight 285 kDa))
NCBI and Uniprot Product Information
NCBI Description
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]
Uniprot Description
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Research Articles on SPTAN1
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Product Notes
The SPTAN1 sptan1 (Catalog #AAA225442) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Spectrin Alpha Chain Antibody with Control Lysate reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Spectrin Alpha Chain can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Western Blotting: Detects a band of approximately 285 kDa in Jurkat cell lysates. Researchers should empirically determine the suitability of the SPTAN1 sptan1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Spectrin Alpha Chain, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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