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Western Blot (WB) (Western blot analysis of cell and mouse liver tissue lysates using Rabbit anti MAPK8/JNK1 antibody)

Rabbit MAPK8 / JNK1 Polyclonal Antibody | anti-MAPK8 antibody

Rabbit anti MAPK8 / JNK1

Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat (Expected from Sequence)
Applications
Immunohistochemistry, Western Blot
Purity
Purified whole serum by affinity chromatography.
Synonyms
MAPK8 / JNK1; Polyclonal Antibody; Rabbit anti MAPK8 / JNK1; MAPK8 / JNK1 Antibody; anti-MAPK8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat (Expected from Sequence)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified whole serum by affinity chromatography.
Form/Format
Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
Sequence Length
427
Applicable Applications for anti-MAPK8 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-Paraffin: 1:100-1:200
WB: 1:500-1:2000
Immunogen
Recombinant human MAPK8/JNK1
Preperation
Antiserum to MAPK8/JNK1 was raised by repeated immunization of rabbits with highly purified antigen.
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of cell and mouse liver tissue lysates using Rabbit anti MAPK8/JNK1 antibody)

Western Blot (WB) (Western blot analysis of cell and mouse liver tissue lysates using Rabbit anti MAPK8/JNK1 antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gastric cancer using Rabbit anti MAPK8/JNK1 antibody at a dilution of 1/100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human gastric cancer using Rabbit anti MAPK8/JNK1 antibody at a dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using Rabbit anti MAPK8/JNK1 antibody at a dilution of 1/100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer using Rabbit anti MAPK8/JNK1 antibody at a dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon using Rabbit anti MAPK8/JNK1 antibody at a dilution of 1/100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon using Rabbit anti MAPK8/JNK1 antibody at a dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate using Rabbit anti MAPK8/JNK1 antibody at a dilution of 1/100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate using Rabbit anti MAPK8/JNK1 antibody at a dilution of 1/100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using Rabbit anti MAPK8/JNK1 antibody at a dilution of 1/100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using Rabbit anti MAPK8/JNK1 antibody at a dilution of 1/100)
Related Product Information for anti-MAPK8 antibody
Rabbit anti MAPK8/JNK1 antibody recognizes mitogen-activated protein kinase 8 (MAPK8), also kown as JNK1 and SAPK1. MAPK8 is a member of the MAPK/JNK family of kinases. MAPK/JNK family members are involved in a number of cellular processes including proliferation, transcription and differentiation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
35,333 Da
NCBI Official Full Name
Mitogen-activated protein kinase 8
NCBI Official Synonym Full Names
mitogen-activated protein kinase 8
NCBI Official Symbol
MAPK8
NCBI Official Synonym Symbols
JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
NCBI Protein Information
mitogen-activated protein kinase 8
UniProt Protein Name
Mitogen-activated protein kinase 8
UniProt Gene Name
MAPK8
UniProt Synonym Gene Names
JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]

Uniprot Description

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973).

Research Articles on MAPK8

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Product Notes

The MAPK8 mapk8 (Catalog #AAA225137) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti MAPK8 / JNK1 reacts with Human, Mouse, Rat (Expected from Sequence) and may cross-react with other species as described in the data sheet. AAA Biotech's MAPK8 / JNK1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-Paraffin: 1:100-1:200 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MAPK8 mapk8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPK8 / JNK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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