Mouse anti-Pig CSF1R Monoclonal Antibody | anti-CSF1R antibody

MOUSE ANTI PIG CSF1R: APC

Cat. Num. AAA224825

• Gene Names: CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• Reactivity: Pig
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CSF1R; Monoclonal Antibody; MOUSE ANTI PIG CSF1R: APC; anti-CSF1R antibody

• Host: Mouse
• Reactivity: Pig
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: ROS8G11
• Specificity: CSF1R
• Form/Format: Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
• Sequence Length: 454

• Applicable Applications for anti-CSF1R antibody: Flow Cytometry (FC/FACS)
• Reactivity Notes: Dog
• Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Preservative Stabilisers: 0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin, 5% Sucrose
• Buffer Solution: Phosphate buffered saline
• Reconstitution: Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
• Preparation and Storage: Prior to reconstitution store at +4 degree C. After reconstitution store at +4 degree C. DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. ; Shelf Life: 18 months from date of reconstitution

Flow Cytometry (FC/FACS)

(Staining of dog bone marrow derived macrophages (BMDMs) using Mouse anti pig CSF1-R, clone ROS 8G11 . Image courtesy of the Roslin Institute)

Flow Cytometry (FC/FACS)

(Staining of pig alveolar macrophages with Mouse anti Pig CSF1R)

Flow Cytometry (FC/FACS)

(Staining of pig alveolar macrophages with Mouse anti Pig CSF1R )

Flow Cytometry (FC/FACS)

(Staining of pig alveolar macrophages with Mouse anti Pig CSF1R followed by FITC conjugated Goat anti Mouse IgG following permeabilization with Leucoperm™ (BUF09))

Related Product Information for anti-CSF1R antibody

Mouse anti Pig CSF1R, clone ROS8G11, recognizes the porcine homologue of human CSF1R, also known as CSF-1R, M-CSF receptor, CD115 and c-fms. CSF1R (CD115) is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceau et al. 2010). CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cell commitment and CSF1R expression appears primarily limited to macrophages (Garceau et al. 2010). The development of the monoclonal antibody ROS8G11 specifically recognizing porcine CSF1R provides an antibody that will assist with research into porcine monocyte and macrophage biology (Moffat et al. 2014). Clone ROS8G11 has been tested for cross reactivity in a limited number of species, and while it does not recognize human or equine CSFR1, further testing is required to conclude if the antibody recognizes CSF1R in other species such as canine.

NCBI and Uniprot Product Information

• NCBI GI #: 125858971
• NCBI GeneID: 1436
• Molecular Weight: 33,248 Da
• NCBI Official Full Name: CSF1R protein, partial
• NCBI Official Synonym Full Names: colony stimulating factor 1 receptor
• NCBI Official Symbol: CSF1R
• NCBI Official Synonym Symbols: FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• NCBI Protein Information: macrophage colony-stimulating factor 1 receptor
• UniProt Protein Name: Macrophage colony-stimulating factor 1 receptor
• Protein Family: Macrophage colony-stimulating factor 1 receptor
• UniProt Gene Name: CSF1R
• UniProt Synonym Gene Names: FMS; CSF-1-R; CSF-1R; M-CSF-R
• UniProt Entry Name: CSF1R_HUMAN

NCBI Description

The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

CSFR: an oncogenic tyrosine kinase receptor for CSF-1 (M-CSF). Drives growth and development of monocytes. Binding of CSF-1 induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins. There are at least five major tyrosine autophosphorylation sites. Two point mutations seen in 10-20% of patients with acute myeloid leukemia, chronic myelomonocytic leukemia or myelodysplasia. One mutation appears to be both somatic and germline, and disrupts Cbl binding and receptor turnover. v-fms lacks the Cbl binding site and causes feline leukemia. Mutations may also develop after chemotherapy for lymphoma. A distinct point mutation was found in some cases of hepatocellular carcinoma and related to increased expression, and another mutation was found in 2 of 40 patients with idiopathic myelofibrosis. Expression is elevated in breast tumors and cell lines, and expression in xenografts and transgenic mice has been correlated with xenograft growth and breast cancer development. Inhibitors: Ki-20227 and other Kit/PDGFR inhibitors.

Protein type: EC 2.7.10.1; Oncoprotein; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; PDGFR family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: cell surface; integral to plasma membrane; intracellular; plasma membrane

Molecular Function: ATP binding; cytokine binding; macrophage colony stimulating factor receptor activity; protein homodimerization activity; protein phosphatase binding

Biological Process: axon guidance; cell proliferation; cytokine and chemokine mediated signaling pathway; forebrain neuron differentiation; hemopoiesis; inflammatory response; innate immune response; intercellular junction maintenance; macrophage differentiation; monocyte differentiation; multicellular organismal development; negative regulation of apoptosis; negative regulation of cell proliferation; olfactory bulb development; osteoclast differentiation; peptidyl-tyrosine phosphorylation; phosphatidylinositol metabolic process; phosphoinositide-mediated signaling; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of osteoclast differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of tyrosine phosphorylation of Stat3 protein; protein amino acid autophosphorylation; regulation of bone resorption; regulation of cell shape; ruffle organization and biogenesis; signal transduction; skeletal muscle development; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Leukoencephalopathy, Diffuse Hereditary, With Spheroids