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Mouse Bu-1a/b Monoclonal Antibody | anti-Bu-1a/b antibody

MOUSE ANTI CHICKEN Bu-1a/b

Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
Bu-1a/b; Monoclonal Antibody; MOUSE ANTI CHICKEN Bu-1a/b; anti-Bu-1a/b antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AV20
Specificity
Clone AV20 recognises Chicken Bu-1a/b, also known as ChL6, a type 1 transmembrane protein.

Bu-1 is a product of two alleles, Bu-1a ad Bu-1b, clone AV20 recognises both alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages. The antigen is absent from erythrocytes, granulocytes and thrombocytes. In addition to clone AV20, clone L22 that specifically recognises the Bu-1a but not Bu-1b is available from MyBioSource.
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5mg/ml (varies by lot)
Sequence Length
335
Applicable Applications for anti-Bu-1a/b antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Immunogen
Bursal cells from day old H.B15 (Bu-1a/b) chickens
Buffer Solution
Target Species
Chicken
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-Bu-1a/b antibody
Clone AV20 recognises Chicken Bu-1a/b, also known as ChL6, a type 1 transmembrane protein. Bu-1 is a product of two alleles, Bu-1a ad Bu-1b, clone AV20 recognises both alleles. The Bu-1 antigen is expressed by chicken B-cells throughout most of their development and by a subset of monocytes and macrophages. The antigen is absent from erythrocytes, granulocytes and thrombocytes. In addition to clone AV20, clone L22 that specifically recognises the Bu-1a but not Bu-1b is available from MyBioSource.

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
36,717 Da
NCBI Official Full Name
B6.2 (Bu-1b)
UniProt Protein Name
B6.2 (Bu-1b)
UniProt Entry Name
Q90747_CHICK

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Product Notes

The Bu-1a/b (Catalog #AAA224663) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Bu-1a/b can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Researchers should empirically determine the suitability of the Bu-1a/b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bu-1a/b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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