Rat TIM-3 Monoclonal Antibody | anti-TIM-3 antibody

RAT ANTI MOUSE TIM-3

Cat. Num. AAA224457

• Gene Names: Havcr2; Tim3; TIM-3; Timd3
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: TIM-3; Monoclonal Antibody; RAT ANTI MOUSE TIM-3; anti-TIM-3 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: RMT3-23
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1 mg/ml (varies by lot)
• Sequence Length: 281

• Applicable Applications for anti-TIM-3 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology Paraffin
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: Tim-3-Ig, consisting of amino acids 1-191 of mouse Tim-3, coupled with the Fc portion of mouse IgG2a.; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Tim-3 transfected LY5178Y cells stained with Rat anti Mouse TIM-3:FITC (MBS224586))

Testing Data #2

(Tim-3 transfected LY5178Y cells stained with Rat anti Mouse TIM-3 (MBS224457))

Testing Data #3

(Tim-3 transfected LY5178Y cells stained with Rat anti Mouse TIM-3:RPE (MBS224712))

Testing Data #4

(Tim-3 transfected LY5178Y cells stained with Rat anti Mouse TIM-3:Low Endotoxin(MBS2130980EL))

Related Product Information for anti-TIM-3 antibody

Rat anti Mouse Tim-3 antibody, clone RMT3-23 specifically recognizes mouse T-cell immunoglobulin mucin 3, a transmembrane glycoprotein and member of the immunoglobulin superfamily, preferentially expressed by differentiated CD4+ Th1 cells and CD8+ Tc1 (cytotoxic) cells, but not by CD4+ Th2 cells or CD8+ Tc2 cells. Tim-3 acts as a negative regulator of Th1-mediated inflammatory diseases including GVHD, type I diabetes, and EAE (experimental autoimmune encephamyelitis), promotes immunological tolerance, and is a receptor for galectin-9, shown to induce cell death of Th1 cells in vitro. Tim-3 is also involved in the regulation of macrophage activation, acts as a phagocytic receptor, and has been shown to recognize apoptotic cells via the IgV domain. Studies have shown thatRat anti Mouse Tim-3 antibody, clone RMT3-23 significantly inhibits the efficient phagocytosis of apoptotic cells by PEMs (peritoneal exudate Mac1+ cells) (Nakayama et al. 2009).

NCBI and Uniprot Product Information

• NCBI GI #: 160333699
• NCBI GeneID: 171285
• NCBI Accession #: NP_599011.2
• NCBI GenBank Nucleotide #: NM_134250.2
• UniProt Accession #: Q8VIM0
• Molecular Weight: 30,934 Da
• NCBI Official Full Name: hepatitis A virus cellular receptor 2 homolog
• NCBI Official Synonym Full Names: hepatitis A virus cellular receptor 2
• NCBI Official Symbol: Havcr2
• NCBI Official Synonym Symbols: Tim3; TIM-3; Timd3
• NCBI Protein Information: hepatitis A virus cellular receptor 2 homolog; HAVcr-2; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIMD-3
• UniProt Protein Name: Hepatitis A virus cellular receptor 2 homolog
• UniProt Gene Name: Havcr2
• UniProt Synonym Gene Names: Tim3; Timd3; HAVcr-2; TIMD-3; TIM-3
• UniProt Entry Name: HAVR2_MOUSE

Uniprot Description

TIM-3: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. Belongs to the immunoglobulin superfamily. TIM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane

Molecular Function: protein binding