Testing Data #1
(Western blot analysis of SK-N-SH human neuroblastoma cell lysate probed with Rabbit anti Human SRPX1 (MBS224279))
Rabbit anti-Mouse, Rat SRPX1 Polyclonal Antibody | anti-SRPX1 antibody
RABBIT ANTI SRPX1
Gene Names
SRPX; DRS; ETX1; SRPX1; HEL-S-83p
Reactivity
Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
SRPX1; Polyclonal Antibody; RABBIT ANTI SRPX1; anti-SRPX1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This item recognises human Sushi-repeat-containing protein X-linked 1 (SRPX1), a 464 amino acid protein involved in autophagy.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
444
Applicable Applications for anti-SRPX1 antibody
ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB)
Application Notes
ELISA: This product is suitable for use in indirect ELISA applications.
Western Blot: This item detects bands of approximately 75kDa SK-N-SH in cell lysates
Immunohistology - Paraffin: Minimum Dilution: 5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.25ug/ml
Western Blot: This item detects bands of approximately 75kDa SK-N-SH in cell lysates
Immunohistology - Paraffin: Minimum Dilution: 5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.25ug/ml
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Preparation
Preparation
Immunogen
A synthetic peptide corresponding to 13 amino acid sequence from near the amino terminus of human SRPX1
Histology Positive Control Tissue
Histology Positive Control Tissue
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Western blot analysis of SK-N-SH human neuroblastoma cell lysate probed with Rabbit anti Human SRPX1 (MBS224279))
Testing Data #1
(Western blot analysis of SK-N-SH human neuroblastoma cell lysate probed with Rabbit anti Human SRPX1 (MBS224279))
Testing Data #2
(Formalin fixed, paraffin embedded human brain stained with Rabbit anti Human SRPX1 (MBS224279) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Testing Data #2
(Formalin fixed, paraffin embedded human brain stained with Rabbit anti Human SRPX1 (MBS224279) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Related Product Information for anti-SRPX1 antibody
MBS224279 recognises human Sushi-repeat-containing protein X-linked 1 (SRPX1), a 464 amino acid protein involved in autophagy.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,382 Da
NCBI Official Full Name
sushi repeat-containing protein SRPX isoform 2
NCBI Official Synonym Full Names
sushi-repeat containing protein, X-linked
NCBI Official Symbol
SRPX
NCBI Official Synonym Symbols
DRS; ETX1; SRPX1; HEL-S-83p
NCBI Protein Information
sushi repeat-containing protein SRPX; epididymis secretory sperm binding protein Li 83p; sushi-repeat-containing protein, X chromosome; sushi-repeat-containing protein, X-linked
UniProt Protein Name
Sushi repeat-containing protein SRPX
UniProt Gene Name
SRPX
UniProt Synonym Gene Names
ETX1
UniProt Entry Name
SRPX_HUMAN
Uniprot Description
SRPX: May be involved in phagocytosis during disk shedding, cell adhesion to cells other than the pigment epithelium or signal transduction. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: Xp21.1
Cellular Component: cell surface; membrane; endoplasmic reticulum; autophagic vacuole
Biological Process: phagolysosome formation; autophagy; cell adhesion
Research Articles on SRPX1
Similar Products
Product Notes
The SRPX1 srpx (Catalog #AAA224279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI SRPX1 reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRPX1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB). ELISA: This product is suitable for use in indirect ELISA applications. Western Blot: This item detects bands of approximately 75kDa SK-N-SH in cell lysates Immunohistology - Paraffin: Minimum Dilution: 5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Western Blotting: Minimum Dilution: 0.25ug/ml. Researchers should empirically determine the suitability of the SRPX1 srpx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRPX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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