Rabbit anti-Mouse, Rat ACTIN BETA Polyclonal Antibody | anti-ACTIN BETA antibody

RABBIT ANTI HUMAN ACTIN BETA (N-TERMINAL)

Cat. Num. AAA224149

• Gene Names: ACTB; BRWS1; PS1TP5BP1
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: ACTIN BETA; Polyclonal Antibody; RABBIT ANTI HUMAN ACTIN BETA (N-TERMINAL); anti-ACTIN BETA antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 375

• Applicable Applications for anti-ACTIN BETA antibody: Immunohistology Frozen, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 40kDa in HeLa cell lysates.; Immunohistology - Frozen: Maximum Dilution: 1.0ug/ml; Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 2.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Histology Positive Control Tissue: HeLa cells; Buffer Solution
• Antiserum Preparation: Antiserum to human Actin beta(N-Terminal) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from HeLa, human epithelial adenocarcinoma cells probed with Rabbit anti Human actin beta (MBS224149) at 0.5(A), 1(B) and 2(C) ug/ml)

Testing Data #2

(Immunocytochemical staining of HeLa cells with Rabbit anti Human actin beta (MBS224149))

Related Product Information for anti-ACTIN BETA antibody

Rabbit anti Human actin beta antibody recognizes human actin beta (ACTB), one of the cytoplasmic actin isoforms, a 41.7 kDa cytoskeletal protein. Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Higher vertebrates express six actin isoforms encoded for by distinct genes. Actin isoforms are highly evolutionarily conserved. Two striated muscle isoforms, alpha skeletal and alpha cardiac, two smooth muscle isoforms, alpha smooth and gamma smooth together with two cytoplasmic isoforms, beta cytoplasmic and gamma cytoplasmic are encoded by genes on chromosomes 1, 15, 10, 2, 7 and 17 respectively. All actin isoforms have highly homologous nucleotide sequences with the heterogeneity most notably in the N terminal region. However, small differences in primary structure here and elsewhere affect the conformational state of the various isoforms and their affinity for a range of actin binding proteins (ABPs). The range of affinities for ABPs suggests that where multiple globular monomeric (G-actin) forms exist at the same site, polymerisation may result in the formation of filamentous (F-actin) enriched for one particular isoform.Defects in actin beta are a cause of juvenile dystonia, a condition that results in movement disorders due to disordered muscle tonicity, abnormal posture, development malformations and sensory hearing loss.

NCBI and Uniprot Product Information

• NCBI GI #: 4501885
• NCBI GeneID: 60
• NCBI Accession #: NP_001092.1
• NCBI GenBank Nucleotide #: NM_001101.3
• UniProt Accession #: P60709; P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
• Molecular Weight: 41,737 Da
• NCBI Official Full Name: actin, cytoplasmic 1
• NCBI Official Synonym Full Names: actin, beta
• NCBI Official Symbol: ACTB
• NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
• NCBI Protein Information: actin, cytoplasmic 1; PS1TP5-binding protein 1; beta cytoskeletal actin
• UniProt Protein Name: Actin, cytoplasmic 1
• UniProt Gene Name: ACTB
• UniProt Entry Name: ACTB_HUMAN

NCBI Description

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; focal adhesion; protein complex; postsynaptic density; cytosol; ribonucleoprotein complex; nucleoplasm; cytoskeleton; membrane; axon; cytoplasm; nuclear chromatin; plasma membrane

Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; Tat protein binding; kinesin binding; nitric-oxide synthase binding; protein kinase binding; ATP binding

Biological Process: axon guidance; intercellular junction assembly and maintenance; establishment and/or maintenance of chromatin architecture; protein folding; retinal homeostasis; 'de novo' posttranslational protein folding; cellular protein metabolic process; substantia nigra development; ephrin receptor signaling pathway; ATP-dependent chromatin remodeling; innate immune response; blood coagulation; vascular endothelial growth factor receptor signaling pathway; cell motility

Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1