Goat ABCB5 Polyclonal Antibody | anti-ABCB5 antibody

GOAT ANTI HUMAN ABCB5

Cat. Num. AAA223834

• Gene Names: ABCB5; ABCB5beta; EST422562; ABCB5alpha
• Applications: ELISA, Western Blot
• Synonyms: ABCB5; Polyclonal Antibody; GOAT ANTI HUMAN ABCB5; anti-ABCB5 antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises human ABCB5 (ATP-binding cassette sub-family B member 5), a P-glycoprotein and member of the conserved ABC transporter family, which acts as an energy-dependent efflux transporter for rhodamine. ; ABCB5 is primarily expressed by skin progenitor cells and shown to act as a regulator of cell fusion and determinant of membrane potential. Studies have revealed the expression of ABCB5 in human malignant melanoma tumours, and in particular its association with CD133+ stem cell phenotype-expressing cells.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 1257

• Applicable Applications for anti-ABCB5 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 80kDa in A431cell lysates; ELISA: Maximum Dilution: 1/128000; Western Blotting: Minimum Dilution: 0.03; Maximum Dilution: 0.1ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Peptide sequence C-QTQHRNTSKKAQ from the internal region of ABCB5 (NP_848654.3); Buffer Solution
• Antiserum Preparation: Antiserum to ABCB5 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-ABCB5 antibody

MBS223834 specifically recognises human ABCB5 (ATP-binding cassette sub-family B member 5), a P-glycoprotein and member of the conserved ABC transporter family, which acts as an energy-dependent efflux transporter for rhodamine. ABCB5 is primarily expressed by skin progenitor cells and shown to act as a regulator of cell fusion and determinant of membrane potential. Studies have revealed the expression of ABCB5 in human malignant melanoma tumours, and in particular its association with CD133+ stem cell phenotype-expressing cells.

NCBI and Uniprot Product Information

• NCBI GI #: 255708477
• NCBI GeneID: 340273
• NCBI Accession #: NP_001157413.1
• NCBI GenBank Nucleotide #: NM_001163941.1
• UniProt Accession #: Q2M3G0; Q2M3I5; Q5I5Q7; Q5I5Q8; Q6KG50; A4D131; A7BKA4; B5MD19; B7WPL1; F8QQP8; F8QQP9; J3KQ04
• Molecular Weight: 89,832 Da
• NCBI Official Full Name: ATP-binding cassette sub-family B member 5 isoform 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 5
• NCBI Official Symbol: ABCB5
• NCBI Official Synonym Symbols: ABCB5beta; EST422562; ABCB5alpha
• NCBI Protein Information: ATP-binding cassette sub-family B member 5; ABCB5 P-gp; ATP-binding cassette protein; P-glycoprotein ABCB5
• UniProt Protein Name: ATP-binding cassette sub-family B member 5
• Protein Family: ABC transporter B family
• UniProt Gene Name: ABCB5
• UniProt Entry Name: ABCB5_HUMAN

NCBI Description

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

Uniprot Description

ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family; Transporter

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: efflux transmembrane transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATP binding

Biological Process: regulation of membrane potential; metabolic process; compound eye corneal lens development; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; cell differentiation; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; transmembrane transport; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent