Sheep FIBRINOGEN Polyclonal Antibody | anti-FGA antibody

SHEEP ANTI HUMAN FIBRINOGEN:FITC

Cat. Num. AAA222660

• Gene Names: FGA; Fib2
• Applications: Immunofluorescence
• Synonyms: FIBRINOGEN; Polyclonal Antibody; SHEEP ANTI HUMAN FIBRINOGEN:FITC; anti-FGA antibody

• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 866

• Applicable Applications for anti-FGA antibody: Immunofluorescence (IF)
• Application Notes: Immunofluorescence: Minimum Dilution: 1/10; Maximum Dilution: 1/100
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to human fibrinogen were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-FGA antibody

Sheep anti Human fibrinogen antibody recognizes human fibrinogen, a complex ~340kDa hetero-hexameric (di-trimeric) glycoprotein consisting of 3 pairs of alpha, beta and gamma chains linked by a series of 29 disulphide bonds (Henschen et al. 1983). The six chains are arranged in such a way that all the N-Terminal ends adjoin to form a central E domain with two trimeric coiled coil structures connecting to outer D domains. Fibrinogen plays an important role in the coagulation process with the D and E domains interacting via the C-Terminal ends of the alpha chains during fibrin clot cross-linking. Sheep anti human fibrinogen antibody shows minimal cross-reactivity with related serum proteins. Fibrinogen has been identified as a ferritin binding protein in the horse (Orino et al. 1993). Sheep anti human fibrinogen antibody has been successfully as a capture reagent for ferritin - anti ferritin IgG complexes in horse plasma to evaluate the antibody response to ferritin by ELISA (Takahashi et al. 2013).

NCBI and Uniprot Product Information

• NCBI GI #: 4503689
• NCBI GeneID: 2243
• NCBI Accession #: NP_000499.1
• NCBI GenBank Nucleotide #: NM_000508.3
• Molecular Weight: 69,757 Da
• NCBI Official Full Name: fibrinogen alpha chain isoform alpha-E preproprotein
• NCBI Official Synonym Full Names: fibrinogen alpha chain
• NCBI Official Symbol: FGA
• NCBI Official Synonym Symbols: Fib2
• NCBI Protein Information: fibrinogen alpha chain; fibrinogen, A alpha polypeptide
• UniProt Protein Name: Fibrinogen alpha chain
• Protein Family: Fibrinogen
• UniProt Gene Name: FGA
• UniProt Entry Name: FIBA_HUMAN

NCBI Description

The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus. [provided by RefSeq, Jul 2008]

Uniprot Description

FGA: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. Defects in FGA are a cause of congenital afibrinogenemia (CAFBN). This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha- dysfibrinogenemias. Defects in FGA are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: extracellular space; cell surface; fibrinogen complex; plasma membrane; extracellular region; cell cortex; vesicle; external side of plasma membrane

Molecular Function: protein binding, bridging; protein binding; cell adhesion molecule binding; structural molecule activity; receptor binding

Biological Process: protein polymerization; platelet activation; extracellular matrix organization and biogenesis; cellular protein complex assembly; positive regulation of heterotypic cell-cell adhesion; platelet degranulation; positive regulation of protein secretion; cell-matrix adhesion; positive regulation of vasoconstriction; innate immune response; blood coagulation; response to calcium ion; signal transduction; positive regulation of exocytosis

Disease: Amyloidosis, Familial Visceral; Afibrinogenemia, Congenital; Dysfibrinogenemia, Congenital