Testing Data #1
(Western blot analysis of mouse kidney lysate probed with Rabbit anti Mouse CAD (MBS221593))
Rabbit CAD Polyclonal Antibody | anti-CAD antibody
RABBIT ANTI MOUSE CAD (aa205-222)
Gene Names
Dffb; CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik
Applications
Immunohistochemistry, Western Blot
Synonyms
CAD; Polyclonal Antibody; RABBIT ANTI MOUSE CAD (aa205-222); anti-CAD antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
CAD (Caspase Activated Deoxyribonuclease) is the murine homologue of human DFF-40.CAD is released and activated by caspase cleavage of ICAD, and eventually causes degradation of nuclear DNA. This item recognises the 40kD CAD in Western blots.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 0.5mg/ml (varies by lot)
Sequence Length
344
Applicable Applications for anti-CAD antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/1000
Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/1000
Perservative Stabilisers
0.02% Sodium Azide
Immunogen
Synthetic peptide corresponding to amino acids 205-222 of mouse CAD
Buffer Solution
Buffer Solution
Antiserum Preparation
Antisera to murine CAD were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Western blot analysis of mouse kidney lysate probed with Rabbit anti Mouse CAD (MBS221593))
Testing Data #1
(Western blot analysis of mouse kidney lysate probed with Rabbit anti Mouse CAD (MBS221593))
Testing Data #2
(Immunohistochemical stainning of mouse kidney with Rabbit anti Mouse CAD (MBS221593))
Testing Data #2
(Immunohistochemical stainning of mouse kidney with Rabbit anti Mouse CAD (MBS221593))
Related Product Information for anti-CAD antibody
CAD (Caspase Activated Deoxyribonuclease) is the murine homologue of human DFF-40. CAD is released and activated by caspase cleavage of ICAD, and eventually causes degradation of nuclear DNA. MBS221593 recognises the 40kD CAD in Western blots.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,449 Da
NCBI Official Full Name
DNA fragmentation factor subunit beta
NCBI Official Synonym Full Names
DNA fragmentation factor, beta subunit
NCBI Official Symbol
Dffb
NCBI Official Synonym Symbols
CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik
NCBI Protein Information
DNA fragmentation factor subunit beta; DFF-40; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor, 40 kD, beta subunit; DNase inhibited by DNA fragmentation factor; caspase-activated DNase; caspase-activated deoxyribonuclease
UniProt Protein Name
DNA fragmentation factor subunit beta
Protein Family
UniProt Gene Name
Dffb
UniProt Synonym Gene Names
Cad; CAD; Caspase-activated DNase; DFF-40
UniProt Entry Name
DFFB_MOUSE
Uniprot Description
DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Deoxyribonuclease; Apoptosis; EC 3.-.-.-
Cellular Component: nuclear chromatin; cytoplasm; nucleolus; intracellular; cytosol; nucleus
Molecular Function: protein binding; nuclease activity; enzyme binding; DNA binding; hydrolase activity; deoxyribonuclease activity; GPI-anchor transamidase activity
Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation
Research Articles on CAD
Similar Products
Product Notes
The CAD dffb (Catalog #AAA221593) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CAD can be used in a range of immunoassay formats including, but not limited to, Immunohistology Paraffin*, Western Blot (WB). Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/1000. Researchers should empirically determine the suitability of the CAD dffb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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