Hamster Matrix Metalloproteinase 7 ELISA Kit | MMP-7 elisa kit

Hamster Matrix Metalloproteinase 7 ELISA Kit

Cat. Num. AAA22159

• Gene Names: MMP7; MMP-7; MPSL1; PUMP-1
• Reactivity: Hamster
• Synonyms: Matrix Metalloproteinase 7; Hamster Matrix Metalloproteinase 7 ELISA Kit; MMP-7 elisa kit

• Reactivity: Hamster
• Specificity: No significant cross-reactivity or interference between Hamster MMP-7 and analogues was observed.
• Sequence Length: 267

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 0.25 ng/ml - 8 ng/ml.
• Sensitivity: 0.1 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated MMP-7 concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster MMP-7 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MMP-7 and MMP-7 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MMP-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MMP-7 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated MMP-7 concentrations in Hamster serum, plasma and other body fluids. Using Purified Hamster MMP-7 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MMP-7 and MMP-7 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MMP-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505219
• NCBI GeneID: 4316
• NCBI Accession #: NP_002414.1
• NCBI GenBank Nucleotide #: NM_002423.3
• UniProt Accession #: P09237; Q9BTK9
• Molecular Weight: 29,677 Da
• NCBI Official Full Name: matrilysin preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 7 (matrilysin, uterine)
• NCBI Official Symbol: MMP7
• NCBI Official Synonym Symbols: MMP-7; MPSL1; PUMP-1
• NCBI Protein Information: matrilysin; matrin; pump-1 protease; uterine matrilysin; uterine metalloproteinase; matrix metalloproteinase-7; matrix metalloproteinase 7 (matrilysin, uterine)
• UniProt Protein Name: Matrilysin
• UniProt Gene Name: MMP7
• UniProt Synonym Gene Names: MPSL1; PUMP1; MMP-7
• UniProt Entry Name: MMP7_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP7: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.23; Secreted; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.2

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: response to drug; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; defense response to Gram-positive bacterium; antibacterial peptide biosynthetic process; proteolysis; defense response to Gram-negative bacterium; antibacterial peptide secretion; regulation of cell proliferation