Rabbit TAP1 Polyclonal Antibody | anti-TAP1 antibody

RABBIT ANTI HUMAN TAP1 (C-TERMINAL)

Cat. Num. AAA221531

• Gene Names: TAP1; APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*0102N
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Synonyms: TAP1; Polyclonal Antibody; RABBIT ANTI HUMAN TAP1 (C-TERMINAL); anti-TAP1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects an epitope within the C-terminal region of human TAP1, also known as transporter associated with antigen processing 1. TAP1 is an 81kDa membrane-associated protein which is a member of the ATP-binding cassette (ABC) transporter superfamily and together with the homologous subunit TAP2 forms the functional TAP complex. ; TAP1 is involved in the transport of antigenic peptides from the cytosol into the endoplasmic reticulum, where they associate with major histocompatibility complex (MHC) class I molecules. These complexes are presented at the cell surface to T-lymphocytes, which identify and remove infected or malignantly transformed cells. ; This item does not cross react with TAP2.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 808

• Applicable Applications for anti-TAP1 antibody: ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 72kDa; ELISA: Maximum Dilution: 1/10000; Western Blotting: Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.01% Sodium Azide (NaN3)
• Immunogen: TAP1 peptide corresponding to the C-terminal of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).; Buffer Solution
• Antiserum Preparation: Antisera to TAP1 (C-terminal) were raised by repeated immunisations of rabbits with highly purified antigen.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-TAP1 antibody

MBS221531 detects an epitope within the C-terminal region of human TAP1, also known as transporter associated with antigen processing 1. TAP1 is an 81kDa membrane-associated protein which is a member of the ATP-binding cassette (ABC) transporter superfamily and together with the homologous subunit TAP2 forms the functional TAP complex. TAP1 is involved in the transport of antigenic peptides from the cytosol into the endoplasmic reticulum, where they associate with major histocompatibility complex (MHC) class I molecules. These complexes are presented at the cell surface to T-lymphocytes, which identify and remove infected or malignantly transformed cells. MBS221531 does not cross react with TAP2.

NCBI and Uniprot Product Information

• NCBI GI #: 9665248
• NCBI GeneID: 6890
• NCBI Accession #: NP_000584.2
• NCBI GenBank Nucleotide #: NM_000593.5
• UniProt Accession #: Q03518; Q16149; Q96CP4
• Molecular Weight: 87,218 Da
• NCBI Official Full Name: antigen peptide transporter 1 isoform 1
• NCBI Official Synonym Full Names: transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
• NCBI Official Symbol: TAP1
• NCBI Official Synonym Symbols: APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*0102N
• NCBI Protein Information: antigen peptide transporter 1; ABC transporter, MHC 1; ATP-binding cassette sub-family B member 2; ATP-binding cassette, sub-family B (MDR/TAP), member 2; peptide supply factor 1; peptide transporter PSF1; peptide transporter TAP1; peptide transporter involved in antigen processing 1; really interesting new gene 4 protein; transporter associated with antigen processing; transporter, ATP-binding cassette, major histocompatibility complex, 1
• UniProt Protein Name: Antigen peptide transporter 1
• Protein Family: Protein
• UniProt Gene Name: TAP1
• UniProt Synonym Gene Names: ABCB2; PSF1; RING4; Y3; APT1; PSF-1
• UniProt Entry Name: TAP1_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]

Uniprot Description

TAP1: a 'transporter associated with antigen processing' (TAP) protein. Member of the ATP binding cassette (ABC) family of transmembrane transporters. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. Defects in TAP1 are a cause of bare lymphocyte syndrome.

Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: endoplasmic reticulum membrane; TAP complex; mitochondrion; membrane; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; protein homodimerization activity; peptide transporter activity; TAP1 binding; peptide antigen binding; MHC class I protein binding; ATPase activity, coupled to transmembrane movement of substances; TAP2 binding; ADP binding; ATP binding; peptide-transporting ATPase activity

Biological Process: peptide transport; intracellular transport of viral proteins in host cell; antigen processing and presentation of peptide antigen via MHC class I; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; defense response; antigen processing and presentation of endogenous peptide antigen via MHC class I; transmembrane transport; cytosol to ER transport

Disease: Bare Lymphocyte Syndrome, Type I