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Rabbit CXCL1 Polyclonal Antibody | anti-CXCL1 antibody

RABBIT ANTI RAT CXCL1

Gene Names
Cxcl1; Gro1; CINC-1
Applications
ELISA, Functional Assay, Western Blot
Synonyms
CXCL1; Polyclonal Antibody; RABBIT ANTI RAT CXCL1; anti-CXCL1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Form/Format
Purified
Purified IgG - lyophilised
Concentration
IgG concentration 1.0 mg/ml after reconstitution. (varies by lot)
Sequence Length
96
Applicable Applications for anti-CXCL1 antibody
ELISA (EIA), Functional Assays (FN), Western Blot (WB)
Application Notes
ELISA: Minimum Dilution: 0.5ug/ml; Maximum Dilution: 2.0ug/ml;
Functional Assays: Minimum Dilution: 5ug/ml; Maximum Dilution: 10ug/ml;
Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml
Reconstitution
Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Preparation
Immunogen
Recombinant rat CXCL1
Buffer Solution
Antiserum Preparation
Antiserum to CXCL1 was raised by repeated immunisation of rabbits with highly purified antigen.
Target Species
Rat
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Related Product Information for anti-CXCL1 antibody
Rabbit anti Rat CXCL1 antibody recognizes rat CXCL1 also known as GRO1 and CINC-1, an 8kD cytokine which promotes neutrophil chemotaxis and degranulation and is expressed predominantly by monocytes after activation, but is also expressed by fibroblasts, endothelial cells, synovial cells and several tumour cell lines.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,249 Da
NCBI Official Full Name
growth-regulated alpha protein
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 1
NCBI Official Symbol
Cxcl1
NCBI Official Synonym Symbols
Gro1; CINC-1
NCBI Protein Information
growth-regulated alpha protein; C-X-C motif chemokine 1; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); cytokine-induced neutrophil chemoattractant 1; platelet-derived growth factor-inducible protein KC
UniProt Protein Name
Growth-regulated alpha protein
UniProt Gene Name
Cxcl1
UniProt Synonym Gene Names
Cinc1; Gro; Scyb1; CINC-1
UniProt Entry Name
GROA_RAT

NCBI Description

acts as a neutrophil chemoattractant; may play a role in acute phase inflammatory response [RGD, Feb 2006]

Uniprot Description

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding

Biological Process: neutrophil chemotaxis; positive regulation of potassium ion transport; response to glucocorticoid stimulus; response to amphetamine; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; response to estradiol stimulus; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; response to heat; response to molecule of bacterial origin; positive regulation of superoxide release; response to gamma radiation; immune response; acute inflammatory response

Research Articles on CXCL1

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Product Notes

The CXCL1 cxcl1 (Catalog #AAA221289) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CXCL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Functional Assays (FN), Western Blot (WB). ELISA: Minimum Dilution: 0.5ug/ml; Maximum Dilution: 2.0ug/ml; Functional Assays: Minimum Dilution: 5ug/ml; Maximum Dilution: 10ug/ml; Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml. Researchers should empirically determine the suitability of the CXCL1 cxcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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