Rabbit IgG Polyclonal Antibody | anti-IgG antibody
RABBIT F(ab')2 ANTI RAT IgG:HRP
Applications
Immunohistochemistry, ELISA, Immunoblot, Western Blot
Synonyms
IgG; Polyclonal Antibody; RABBIT F(ab')2 ANTI RAT IgG:HRP; anti-IgG antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
This item recognises the heavy and light chains of rat IgG1, IgG2a, IgG2b and IgG2c. This product also reacts with the light chains of rat IgM as demonstrated by ELISA.
Form/Format
HRP
F(ab')2 fragment of IgG conjugated to Horseradish Peroxidase (HRP) - liquid
F(ab')2 fragment of IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Sequence Length
322
Applicable Applications for anti-IgG antibody
Immunohistology Frozen, ELISA (EIA), Immunoblotting (IB)
Application Notes
Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/200;
ELISA: Minimum Dilution: 1/4000; Maximum Dilution: 1/8000;
Immunoblotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/2000
ELISA: Minimum Dilution: 1/4000; Maximum Dilution: 1/8000;
Immunoblotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/2000
Perservative Stabilisers
50% Glycerol
Immunogen
Rat IgG
Buffer Solution
Buffer Solution
Antiserum Preparation
Antisera to rat IgG was raised by repeated immunisations of rabbits with rat IgG paraproteins. The preparation was purified by affinity chromatography on rat IgG covalently linked to agarose, and gel filtration chromatography of pepsin digested antibody.
Target Species
Rat
Preparation and Storage
Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life: 18 months from the date of despatch.
Shelf Life: 18 months from the date of despatch.
Related Product Information for anti-IgG antibody
MBS220364 recognises the heavy and light chains of rat IgG1, IgG2a, IgG2b and IgG2c. This product also reacts with the light chains of rat IgM as demonstrated by ELISA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
35,186 Da
NCBI Official Full Name
Ig gamma-2A chain C region, partial
NCBI Official Symbol
LOC679045
UniProt Protein Name
Ig gamma-2A chain C region
Protein Family
UniProt Gene Name
Igg-2a
UniProt Entry Name
IGG2A_RAT
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Product Notes
The IgG igg-2a (Catalog #AAA220364) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Immunoblotting (IB). Immunohistology - Frozen: Minimum Dilution: 1/50; Maximum Dilution: 1/200; ELISA: Minimum Dilution: 1/4000; Maximum Dilution: 1/8000; Immunoblotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/2000. Researchers should empirically determine the suitability of the IgG igg-2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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