Mouse TLR7 Monoclonal Antibody | anti-TLR7 antibody

MOUSE ANTI HUMAN TLR7

Cat. Num. AAA214463

• Gene Names: TLR7; TLR7-like
• Applications: ELISA, Western Blot
• Synonyms: TLR7; Monoclonal Antibody; MOUSE ANTI HUMAN TLR7; anti-TLR7 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4F4
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 1049

• Applicable Applications for anti-TLR7 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 121 kDa in Ramos cell lysates.; Western Blotting: Minimum Dilution: 1/1000; Maximum Dilution: 1/2000
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3) ; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Western blot analysis of TLR7 expression against recombinant TLR7 (immunogen) using Mouse anti Human TLR7)

Related Product Information for anti-TLR7 antibody

Mouse anti Human TLR7, clone 4F4 recognizes human TLR7 (toll-like receptor 7), a 121 kDa type I transmembrane protein belonging to the toll-like receptor (TLR) family. TLR7is predominantly expressed in the spleen, lung and placenta (Chuang et al. 2000). TLRs are highly conserved proteins which play a key role in activation of innate immunity, through the recognition of distinct molecular patterns expressed by infectious agents. TLR7 recognizes and interacts with single-stranded RNA (ssRNA) derived from viruses such as influenza, HIV-1 (human immunodeficiency virus type I) and Sendai, and some siRNAs, resulting in the activation of the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway, and the production of proinflammatory signaling molecules (Diebold et al. 2004)

NCBI and Uniprot Product Information

• NCBI GI #: 7706093
• NCBI GeneID: 51284
• NCBI Accession #: NP_057646.1
• NCBI GenBank Nucleotide #: NM_016562.3
• UniProt Accession #: Q9NYK1; Q9NR98; D1CS69
• Molecular Weight: 120,922 Da
• NCBI Official Full Name: toll-like receptor 7
• NCBI Official Synonym Full Names: toll-like receptor 7
• NCBI Official Symbol: TLR7
• NCBI Official Synonym Symbols: TLR7-like
• NCBI Protein Information: toll-like receptor 7; toll-like receptor 7-like
• UniProt Protein Name: Toll-like receptor 7
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR7
• UniProt Entry Name: TLR7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR7: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1. Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; lysosome; endoplasmic reticulum; cytoplasm; integral to membrane; plasma membrane; endosome membrane; receptor complex; endosome

Molecular Function: siRNA binding; single-stranded RNA binding; transmembrane receptor activity; double-stranded RNA binding; drug binding

Biological Process: microglial cell activation; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; positive regulation of NF-kappaB import into nucleus; positive regulation of interferon-alpha biosynthetic process; toll-like receptor 7 signaling pathway; positive regulation of chemokine production; positive regulation of interleukin-8 production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; inflammatory response; defense response to virus; positive regulation of interferon-gamma biosynthetic process; positive regulation of inflammatory response