Mouse PI-9 Monoclonal Antibody | anti-PI-9 antibody

MOUSE ANTI HUMAN PI-9

Cat. Num. AAA212867

• Gene Names: SERPINB9; PI9; CAP3; PI-9; CAP-3
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: PI-9; Monoclonal Antibody; MOUSE ANTI HUMAN PI-9; anti-PI-9 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7D8
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 376

• Applicable Applications for anti-PI-9 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/100; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.; Immunohistology - Paraffin: Maximum Dilution: 1/50; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Perservative Stabilisers: 0.05% Sodium Azide (NaN3) ; Preparation
• Immunogen: Recombinant PI-9 produced in P. pastoris.; Histology Positive Control Tissue
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-PI-9 antibody

Mouse anti Human PI-9 antibody, clone 7D8 recognizes human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a ~42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 has been reported to work in western blotting applications. MyBioSource recommend the use of MBS212867 for this purpose. Clone 7D8 is suitable for use in indirect Immunofluorescence (Hirst et al. 2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4758906
• NCBI GeneID: 5272
• NCBI Accession #: NP_004146.1
• NCBI GenBank Nucleotide #: NM_004155.5
• UniProt Accession #: P50453; Q5TD03; B2RBW3
• Molecular Weight: 42,404 Da
• NCBI Official Full Name: serpin B9
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade B (ovalbumin), member 9
• NCBI Official Symbol: SERPINB9
• NCBI Official Synonym Symbols: PI9; CAP3; PI-9; CAP-3
• NCBI Protein Information: serpin B9; cytoplasmic antiproteinase 3; peptidase inhibitor 9; protease inhibitor 9 (ovalbumin type); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9; serpin peptidase inhibitor, clade B, member 9
• UniProt Protein Name: Serpin B9
• UniProt Gene Name: SERPINB9
• UniProt Synonym Gene Names: PI9; CAP-3; CAP3; PI-9
• UniProt Entry Name: SPB9_HUMAN

NCBI Description

This gene encodes a member of the serine protease inhibitor family which are also known as serpins. The encoded protein belongs to a subfamily of intracellular serpins. This protein inhibits the activity of the effector molecule granzyme B. Overexpression of this protein may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

SERPINB9: Granzyme B inhibitor. Belongs to the serpin family. Ov-serpin subfamily.

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: extracellular space; membrane; cytoplasm; intracellular; cytosol; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protease binding; caspase inhibitor activity

Biological Process: mast cell mediated immunity; response to bacterium; immune response; negative regulation of caspase activity; negative regulation by symbiont of host apoptosis; protection from natural killer cell mediated cytotoxicity; negative regulation of apoptosis