Mouse anti-Feline CORONAVIRUS Monoclonal Antibody

MOUSE ANTI FELINE CORONAVIRUS

Reactivity

Feline

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry, ELISA, Western Blot, Immunofluorescence

Synonyms

CORONAVIRUS; Monoclonal Antibody; MOUSE ANTI FELINE CORONAVIRUS; anti-CORONAVIRUS antibody

Ordering
Cat. Num. AAA212561 For Research Use Only!

Host

Mouse

Reactivity

Feline

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

FIPV3-70

Specificity

Mouse anti Feline Coronavirus antibody, clone FIPV3-70 reacts with feline infectious peritonitis virus (FIPV) type 1 and 2 and is known to be specific for the nucleocapsid. It is also known to react with canine coronavirus (CCV), pig coronavirus transmissible gastroenteritis virus (TGEV) and ferret coronavirus. Some specific activity has been detected against bovine coronavirus (BCV). Mouse anti Feline Coronavirus antibody, clone FIPV3-70 exhibits negative reactivity against Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcivirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine Parvovirus and Canine Parainfluenza virus.

Form/Format

Purified IgG - liquid

Concentration

IgG concentration 1.0 mg/ml (varies by lot)

Applicable Applications for anti-CORONAVIRUS antibody

Flow Cytometry (FC), Immunohistochemistry - Frozen (IHC-Fr), Immunohistochemistry - Paraffin (IHC-P)*, ELISA, Western Blotting (WB), Immunofluorescence (IF)

Application Notes

WB: Under reducing conditions, MBS212561 detects a specific band of 50-56 kDa that represents the nucleocapsid.
MBS212561 detects specific bands at 56 and 46 kDa in reducing gels with CCV as antigen.

*IHC-P: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with appropriate negative/positive controls.

Target Species

Viral

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilizers

<0.1% Sodium Azide (NaN₃)

Immunogen

Coronavirus cocktail.

Preparation and Storage

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

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Product Notes

The CORONAVIRUS (Catalog #AAA212561) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI FELINE CORONAVIRUS reacts with Feline and may cross-react with other species as described in the data sheet. AAA Biotech's CORONAVIRUS can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunohistochemistry - Frozen (IHC-Fr), Immunohistochemistry - Paraffin (IHC-P)*, ELISA, Western Blotting (WB), Immunofluorescence (IF). WB: Under reducing conditions, MBS212561 detects a specific band of 50-56 kDa that represents the nucleocapsid. MBS212561 detects specific bands at 56 and 46 kDa in reducing gels with CCV as antigen. *IHC-P: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with appropriate negative/positive controls. Researchers should empirically determine the suitability of the CORONAVIRUS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CORONAVIRUS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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Mouse anti-Feline CORONAVIRUS Monoclonal Antibody