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SDS-Page (SDS-PAGE)

Alkaline Phosphatase, Placental (ALPP) Recombinant Protein | ALPP recombinant protein

Recombinant Alkaline Phosphatase, Placental (ALPP)

Gene Names
ALPP; ALP; PALP; PLAP; PLAP-1
Applications
SDS-Page, Western Blot
Purity
Greater than 90% by SDS-PAGE
Synonyms
Alkaline Phosphatase; Placental (ALPP); Recombinant Alkaline Phosphatase; ALPP/Alkaline Phosphatase; Placental; PLAP1; ALP; ALPG; ALPPL; GCAP; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; ALPP recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Sequence
Arg249~Met484
Applicable Applications for ALPP recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Tag
N-terminal His Tag
Expression System
Prokaryotic expression
Organism Species
Human
Isoelectric Point
5.7
Subcellular Location
Membrane
Reconstitution
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

SDS-Page

(SDS-PAGE)

SDS-Page (SDS-PAGE)
Product Categories/Family for ALPP recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
250
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57,954 Da
NCBI Official Full Name
alkaline phosphatase, placental type preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, placental
NCBI Official Symbol
ALPP
NCBI Official Synonym Symbols
ALP; PALP; PLAP; PLAP-1
NCBI Protein Information
alkaline phosphatase, placental type; Regan isozyme; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme
UniProt Protein Name
Alkaline phosphatase, placental type
Protein Family
UniProt Gene Name
ALPP
UniProt Synonym Gene Names
PLAP; PLAP-1
UniProt Entry Name
PPB1_HUMAN

NCBI Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ALPP: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Protein type: Cell surface; Membrane protein, integral; Cofactor and Vitamin Metabolism - folate biosynthesis; Phosphatase (non-protein); Motility/polarity/chemotaxis; Membrane protein, GPI anchor; EC 3.1.3.1

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: cell surface; plasma membrane; integral to membrane

Molecular Function: zinc ion binding; alkaline phosphatase activity; magnesium ion binding

Biological Process: dephosphorylation

Research Articles on ALPP

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Product Notes

The ALPP alpp (Catalog #AAA2125475) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Alkaline Phosphatase, Placental (ALPP) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the ALPP alpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Arg249~Met 484. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase, Placental (ALPP), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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