Human Leucine Rich Repeat Containing G Protein Coupled Receptor 5 (LGR5) ELISA Kit | LGR5 elisa kit

Human Leucine Rich Repeat Containing G Protein Coupled Receptor 5 (LGR5)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2125300

• Gene Names: LGR5; FEX; HG38; GPR49; GPR67; GRP49
• Reactivity: Human
• Applications: ELISA
• Synonyms: Leucine Rich Repeat Containing G Protein Coupled Receptor 5 (LGR5); Human Leucine Rich Repeat Containing G Protein Coupled Receptor 5 (LGR5)(Sandwich ELISA) ELISA Kit; ELISA Kit for Leucine Rich Repeat Containing G Protein Coupled Receptor 5 (LGR5); LGR5/Leucine Rich Repeat Containing G Protein Coupled Receptor 5; FEX; GPR49; GPR67; HG38; G Protein-Coupled Receptor 49; LGR5 elisa kit

• Reactivity: Human
• Specificity: Reactive with Human Leucine Rich Repeat Containing G Protein Coupled Receptor 5 (LGR5); This assay has high sensitivity and excellent specificity for detection of Leucine Rich Repeat Containing G Protein Coupled Receptor 5 (LGR5). No significant cross-reactivity or interference between Leucine Rich Repeat Containing G Protein Coupled Receptor 5 (LGR5) and analogues was observed.

• Applicable Applications for LGR5 elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.061ng/mL
• Detection Method: Double-antibody Sandwich
• Assay Length: 3 hours
• Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Rich Repeat Containing G Protein Coupled Receptor 5 (LGR5) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Rich Repeat Containing G Protein Coupled Receptor 5 (LGR5) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100.; Intra-Assay: CV<10%.; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroxine Binding Globulin (TBG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroxine Binding Globulin (TBG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroxine Binding Globulin (TBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thyroxine Binding Globulin (TBG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 50ul Stop Solution. Read at 450nm immediately.
• Preparation and Storage: Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life); The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for LGR5 elisa kit

Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 469608414
• NCBI GeneID: 8549
• NCBI Accession #: NP_001264155.1
• NCBI GenBank Nucleotide #: NM_001277226.1
• UniProt Accession #: O75473; Q4VAM0; Q4VAM2; Q9UP75; D8MCT0
• Molecular Weight: 99998 MW
• NCBI Official Full Name: leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2
• NCBI Official Synonym Full Names: leucine rich repeat containing G protein-coupled receptor 5
• NCBI Official Symbol: LGR5
• NCBI Official Synonym Symbols: FEX; HG38; GPR49; GPR67; GRP49
• NCBI Protein Information: leucine-rich repeat-containing G-protein coupled receptor 5
• UniProt Protein Name: Leucine-rich repeat-containing G-protein coupled receptor 5
• Protein Family: Leucine-rich repeat-containing G-protein coupled receptor
• UniProt Gene Name: LGR5
• UniProt Synonym Gene Names: GPR49; GPR67

NCBI Description

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.