ATPase, H+ Transporting, Mitochondrial F1 Complex Delta Polypeptide Recombinant Protein | ATP5d recombinant protein

Recombinant ATPase, H+ Transporting, Mitochondrial F1 Complex Delta Polypeptide (ATP5d)

Cat. Num. AAA2123782

• Gene Names: ATP5F1D; ATP5D; MC5DN5
• Applications: SDS-Page, Western Blot
• Purity: >95%
• Synonyms: ATPase; H+ Transporting; Mitochondrial F1 Complex Delta Polypeptide; Recombinant ATPase; Mitochondrial F1 Complex Delta Polypeptide (ATP5d); F-ATPase delta subunit; ATP5d recombinant protein

• Host: E Coli
• Purity/Purification: >95%
• Form/Format: Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Met1~Glu168
• Sequence Length: 168

• Applicable Applications for ATP5d recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Human
• Tag: N-terminal His Tag
• Subcellular Location: Secreted
• Isoelectric Point: 5.3
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for ATP5d recombinant protein

Signal transduction

NCBI and Uniprot Product Information

• NCBI GI #: 50345991
• NCBI GeneID: 513
• NCBI Accession #: NP_001001975.1
• NCBI GenBank Nucleotide #: NP_001001975.1
• UniProt Accession #: P30049
• Molecular Weight: Predicted Molecular Mass: 21.2kDa; Accurate Molecular Mass: 23kDa
• NCBI Official Full Name: ATP synthase subunit delta, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase F1 subunit delta
• NCBI Official Symbol: ATP5F1D
• NCBI Official Synonym Symbols: ATP5D; MC5DN5
• NCBI Protein Information: ATP synthase subunit delta, mitochondrial
• UniProt Protein Name: ATP synthase subunit delta, mitochondrial
• UniProt Gene Name: ATP5D
• UniProt Entry Name: ATPD_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP5D: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Belongs to the ATPase epsilon chain family.

Protein type: Energy Metabolism - oxidative phosphorylation; Hydrolase; Mitochondrial; EC 3.6.3.14

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex

Molecular Function: transporter activity; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; transmembrane transporter activity; ADP binding; hydrogen ion transporting ATPase activity, rotational mechanism; ATP binding

Biological Process: cellular metabolic process; ATP synthesis coupled proton transport; response to copper ion; ATP biosynthetic process; mitochondrial ATP synthesis coupled proton transport; oxidative phosphorylation