Human Relaxin 2 (RLN2) ELISA Kit | RLN2 elisa kit

Human Relaxin 2 (RLN2) ELISA Kit

Cat. Num. AAA2122118

• Gene Names: RLN2; H2; RLXH2; H2-RLX; bA12D24.1.1; bA12D24.1.2
• Reactivity: Human
• Synonyms: Relaxin 2 (RLN2); Human Relaxin 2 (RLN2) ELISA Kit; H2; RLXH2; Relaxin H2; Prorelaxin H2; Relaxin; Ovarian; Of Pregnancy; RLN2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Relaxin 2 (RLN2).; No significant cross-reactivity or interference between Relaxin 2 (RLN2) and analogues was observed.
• Sequence Length: 117

• Assay Type: Double-antibody Sandwich
• Sample: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Dectection Range: 7.8-500pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 3.1pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Relaxin 2 (RLN2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Relaxin 2 (RLN2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RLN2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Relaxin 2 (RLN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Relaxin 2 (RLN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Relaxin 2 (RLN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Relaxin 2 (RLN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RLN2 elisa kit

Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 20143935
• NCBI GeneID: 6019
• NCBI Accession #: NP_005050.2
• NCBI GenBank Nucleotide #: NP_005050.2
• UniProt Accession #: P04090
• NCBI Official Full Name: prorelaxin H2 isoform 2
• NCBI Official Synonym Full Names: relaxin 2
• NCBI Official Symbol: RLN2
• NCBI Official Synonym Symbols: H2; RLXH2; H2-RLX; bA12D24.1.1; bA12D24.1.2
• NCBI Protein Information: prorelaxin H2
• UniProt Protein Name: Prorelaxin H2
• Protein Family: Prorelaxin
• UniProt Gene Name: RLN2
• UniProt Entry Name: REL2_HUMAN

NCBI Description

This gene encodes a member of the relaxin subfamily and insulin superfamily of peptide hormones. In humans there are three non-allelic relaxin genes. This gene encodes multiple protein isoforms, at least one of which undergoes proteolytic processing. This processing generates relaxin A and B chains that are linked by disulfide bonds to form the mature peptide hormone. This hormone plays a role in the male and female reproductive systems and was initially noted for its role in pregnancy. This protein also plays broader roles in the cardiovascular system, including in the regulation of blood pressure and control of heart rate, and data from animal models shows that this protein may have anti-fibrotic and cardioprotective effects. [provided by RefSeq, Jul 2016]

Uniprot Description

RLN2: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: female pregnancy