Mouse C3a Monoclonal Antibody | anti-C3a antibody

MOUSE ANTI HUMAN C3a

Cat. Num. AAA211400

• Gene Names: C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
• Reactivity: Guinea Pig, Rabbit; Antibody reactivity and working conditions may vary between species.
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified; Purified IgG prepared by afinnity chromatography on Protein A.
• Synonyms: C3a; Monoclonal Antibody; MOUSE ANTI HUMAN C3a; anti-C3a antibody

• Host: Mouse
• Reactivity: Guinea Pig, Rabbit; Antibody reactivity and working conditions may vary between species.
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: H13
• Purity/Purification: Purified; Purified IgG prepared by afinnity chromatography on Protein A.
• Form/Format: Purified IgG- Lyophilized
• Concentration: IgG concentration 50 ug/ml after reconstitution. (varies by lot)
• Sequence Length: 1663

• Applicable Applications for anti-C3a antibody: Immunohistology-Frozen, ELISA, Western Blot
• Application Notes: Immunohistology-Frozen 1/10
• Immunogen: Human complement component C3
• Reconstitution: Reconstitute with 1.0ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
• Perservative Stabilisers: 0.09% Sodium Azide ,0.5% Bovine Serum Albumin.
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4°C. After reconstitution store at 4°C or at -20° C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Related Product Information for anti-C3a antibody

Mouse anti Human C3a antibody, clone H13 recognizes human C3a anaphylatoxin, a cleavage product of human complement component C3. C3a increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. Mouse anti Human C3a antibody, clone H13 recognizes a C3a-determinant also present on native C3. Clone H13 allows discrimination of ubiquitous or non-specifically bound native C3 or C3a from biologically active C3b fragment, neither C3b or C5 components are recognized by clone H13 (Burger et al. 1987). Mouse anti Human C3a antibody, clone H13 is reported to block C3a functional activity (Hartmann et al. 1997).

NCBI and Uniprot Product Information

• NCBI GI #: 115298678
• NCBI GeneID: 718
• NCBI Accession #: NP_000055.2
• NCBI GenBank Nucleotide #: NM_000064.3
• UniProt Accession #: P01024; A7E236
• NCBI Official Full Name: complement C3
• NCBI Official Synonym Full Names: complement component 3
• NCBI Official Symbol: C3
• NCBI Official Synonym Symbols: ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
• NCBI Protein Information: complement C3; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; acylation-stimulating protein cleavage product; complement component C3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; prepro-C3
• UniProt Protein Name: Complement C3
• Protein Family: C3a anaphylatoxin chemotactic receptor
• UniProt Gene Name: C3
• UniProt Synonym Gene Names: CPAMD1; C3bc; ASP
• UniProt Entry Name: CO3_HUMAN

NCBI Description

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. A peptide (C3a) derived from the encoded protein has antimicrobial activity, so people with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq, Nov 2014]

Uniprot Description

C3: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Defects in C3 are the cause of complement component 3 deficiency (C3D). A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis. Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin- containing structure known as Bruch membrane. Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.

Protein type: Secreted, signal peptide; Secreted; Inhibitor

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: protein binding; endopeptidase inhibitor activity; C5L2 anaphylatoxin chemotactic receptor binding; receptor binding

Biological Process: regulation of immune response; complement activation, alternative pathway; signal transduction; fatty acid metabolic process; complement activation; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; positive regulation of G-protein coupled receptor protein signaling pathway; positive regulation of type IIa hypersensitivity; regulation of complement activation; innate immune response; immune response; positive regulation of protein amino acid phosphorylation; inflammatory response; complement activation, classical pathway

Disease: Complement Component 3 Deficiency, Autosomal Recessive; Hemolytic Uremic Syndrome, Atypical, Susceptibility To, 5; Macular Degeneration, Age-related, 9