WB (Western Blot)
(Detection of human and mouse PTEN by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PTEN antibody AAA211096 (lot AAA211096-1) used for WB at 0.04 ug (A) and 1 ug/ml (B) and used for immunoprecipitation at 3 ug/mg lysate (B). PTEN was also immunoprecipitated by rabbit anti-PTEN antibodies BL2788, BL2789, and Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
Rabbit anti-Human, Mouse PTEN Polyclonal Antibody | anti-PTEN antibody
Rabbit anti-PTEN Antibody, Affinity Purified
Gene Names
PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PTEN, Antibody; Rabbit anti-PTEN Antibody, Affinity Purified; mutated in multiple advanced cancers 1; mitochondrial phosphatase and tensin protein alpha; TEP1; PTENbeta; PTEN1; protein tyrosine phosphatase; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatase and tensin-like protein; Phosphatase and tensin homolog; 10q23del; mitochondrial PTENalpha; MHAM; GLM2; DEC; CWS1; BZS; MMAC1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatase and tensin homolog; anti-PTEN antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PTEN antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Dog
Immunogen
Between 353 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse PTEN by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PTEN antibody AAA211096 (lot AAA211096-1) used for WB at 0.04 ug (A) and 1 ug/ml (B) and used for immunoprecipitation at 3 ug/mg lysate (B). PTEN was also immunoprecipitated by rabbit anti-PTEN antibodies BL2788, BL2789, and Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human and mouse PTEN by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PTEN antibody AAA211096 (lot AAA211096-1) used for WB at 0.04 ug (A) and 1 ug/ml (B) and used for immunoprecipitation at 3 ug/mg lysate (B). PTEN was also immunoprecipitated by rabbit anti-PTEN antibodies BL2788, BL2789, and Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human PTEN by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PTEN (Cat. No. AAA211096 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human PTEN by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PTEN (Cat. No. AAA211096 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-PTEN antibody
PTEN (phosphatase and tensin homolog) is a phosphatidylinositol-3,4,5-triphosphate 3-phosphatase that has been identified as a tumor suppressor. PTEN is a dual specificity tyrosine and serine/threonine phosphatase that preferentially dephosphorylates phosphoinositide substrates and negatively regulates the AKT/PKB signaling pathway. Through its action, PTEN has been shown to modulate cell cycle progression, cell survival, cell migration, cell adhesion, and insulin signaling.
Product Categories/Family for anti-PTEN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,166 Da
NCBI Official Full Name
phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
NCBI Official Synonym Full Names
phosphatase and tensin homolog
NCBI Official Symbol
PTEN
NCBI Official Synonym Symbols
BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del
NCBI Protein Information
phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatase and tensin-like protein; mutated in multiple advanced cancers 1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; phosphatidyli
UniProt Protein Name
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
UniProt Gene Name
PTEN
UniProt Synonym Gene Names
MMAC1; TEP1
UniProt Entry Name
PTEN_HUMAN
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Product Notes
The PTEN pten (Catalog #AAA211096) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PTEN Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PTEN can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PTEN pten for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTEN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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