Typical Testing Data/Standard Curve (for reference only)
Human Activating Natural Killer Receptor P30 (NKp30/NCR3) ELISA Kit | NKp30/NCR3 elisa kit
Human Activating Natural Killer Receptor P30 (NKp30/NCR3) ELISA Kit
Gene Names
NCR3; 1C7; MALS; CD337; LY117; NKp30
Reactivity
Human
Synonyms
Activating Natural Killer Receptor P30 (NKp30/NCR3); Human Activating Natural Killer Receptor P30 (NKp30/NCR3) ELISA Kit; CD337; 1C7; LY117; NKp30; Lymphocyte Antigen 117; Activating natural killer receptor p30; Natural killer cell p30-related protein; NKp30/NCR3 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of NIS. No significant cross-reactivity or interference between NIS and analogues was observed.
Sequence Length
190
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Human Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.059ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NIS were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NIS were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NKp30/NCR3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NIS in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to NIS. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NIS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NIS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NIS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to NIS. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NIS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NIS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NIS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for NKp30/NCR3 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,640 Da
NCBI Official Full Name
natural cytotoxicity triggering receptor 3 isoform c
NCBI Official Synonym Full Names
natural cytotoxicity triggering receptor 3
NCBI Official Symbol
NCR3
NCBI Official Synonym Symbols
1C7; MALS; CD337; LY117; NKp30
NCBI Protein Information
natural cytotoxicity triggering receptor 3
UniProt Protein Name
Natural cytotoxicity triggering receptor 3
UniProt Gene Name
NCR3
UniProt Synonym Gene Names
1C7; LY117; NK-p30; NKp30
NCBI Description
The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells. The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor. A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]
Uniprot Description
Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1. Stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.
Research Articles on NKp30/NCR3
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Product Notes
The Human NKp30/NCR3 ncr3 (Catalog #AAA2104862) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2104862 ELISA Kit recognizes Human NKp30/NCR3. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the NKp30/NCR3 ncr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Activating Natural Killer Receptor P30 (NKp30/NCR3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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