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Testing Data (Sandwich ELISA analysis of Human G-CSG using Rat anti human G-CSF (MBS211289) as a capture reagent and biotinylated Rat anti Human G-CSF as a detection reagent with recombinant human G-CSF as target antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Human serum (orange) and plasma (green) samples are tested undiluted)

Rat G-CSF Monoclonal Antibody | anti-G-CSF antibody

RAT ANTI HUMAN G-CSF:Biotin

Gene Names
CSF3; GCSF; CSF3OS; C17orf33
Applications
ELISA
Synonyms
G-CSF; Monoclonal Antibody; RAT ANTI HUMAN G-CSF:Biotin; anti-G-CSF antibody
Ordering
For Research Use Only!
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
BVD11-37G10
Form/Format
Biotin
Purified IgG conjugated to Biotin - liquid.
Concentration
Approximate Protein Concentration: 0.5 mg/ml (varies by lot)
Sequence Length
171
Applicable Applications for anti-G-CSF antibody
ELISA (EIA)
Perservative Stabilisers
0.1% Sodium Azide (NaN3)
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from the date of despatch.

Testing Data

(Sandwich ELISA analysis of Human G-CSG using Rat anti human G-CSF (MBS211289) as a capture reagent and biotinylated Rat anti Human G-CSF as a detection reagent with recombinant human G-CSF as target antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Human serum (orange) and plasma (green) samples are tested undiluted)

Testing Data (Sandwich ELISA analysis of Human G-CSG using Rat anti human G-CSF (MBS211289) as a capture reagent and biotinylated Rat anti Human G-CSF as a detection reagent with recombinant human G-CSF as target antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Human serum (orange) and plasma (green) samples are tested undiluted)
Related Product Information for anti-G-CSF antibody
Rat anti Human G-CSF antibody, clone BVD11-37G10 recognizes human granulocyte colony stimulating factor (G-CSF), also known as Pluripoietin. In addition Filgrastim and Lenograstim are used to describe recombinant human G-CSF preparations. Human G-CSF is a 207 amino acid, ~20kDa O-linked glycoprotein belonging to the IL-6 superfamily. There are potentially three isoforms formed by alternative splicing, Rat anti Human G-CSF antibody, clone BVD13-3A5 binds the canonical isoform-1, binding to other isoforms has not been assessed (UniProt: P09919). G-CSF is produced by activated macrophages, monocytes and neutrophils. In addition it can be produced by fibroblasts and endothelial cells (Zsebo et al. 1988). G-CSF stimulates production of cells belonging to the granulocytic lineage in the bone marrow and is an activating agent for mature neutrophilic granulocytes (Scapini et al. 2003) to produce other cytokines including CD257 (BAFF), operating through binding to the G-CSF receptor CD114. Biotinylated Rat anti Human G-CSF antibody, clone BVD11-37G10 has been successfully evaluated as a detection reagent in a highly sensitive sandwich ELISA assay with biotinylated Rat anti Human G-CSF, clone BVD11-3A5 as a capture reagent and recombinant human G-CSF as antigen.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,434 Da
NCBI Official Full Name
granulocyte colony-stimulating factor isoform a
NCBI Official Synonym Full Names
colony stimulating factor 3 (granulocyte)
NCBI Official Symbol
CSF3
NCBI Official Synonym Symbols
GCSF; CSF3OS; C17orf33
NCBI Protein Information
granulocyte colony-stimulating factor; filgrastim; granulocyte-colony stimulating factor; lenograstim; pluripoietin
UniProt Protein Name
Granulocyte colony-stimulating factor
UniProt Gene Name
CSF3
UniProt Synonym Gene Names
C17orf33; GCSF; G-CSF
UniProt Entry Name
CSF3_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Cell cycle regulation; Secreted

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space

Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; granulocyte differentiation; positive regulation of protein binding; positive regulation of myeloid cell differentiation; positive regulation of actin filament polymerization; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of peptidyl-serine phosphorylation

Research Articles on G-CSF

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Product Notes

The G-CSF csf3 (Catalog #AAA210293) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's G-CSF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the G-CSF csf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G-CSF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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