Rat Protein Kinase, AMP Activated Alpha 1 (PRKAa1) ELISA Kit | PRKAa1 elisa kit

Rat Protein Kinase, AMP Activated Alpha 1 (PRKAa1) ELISA Kit

Cat. Num. AAA2097915

• Gene Names: PRKAA1; AMPK; AMPKa1
• Reactivity: Rat
• Synonyms: Protein Kinase; AMP Activated Alpha 1 (PRKAa1); Rat Protein Kinase; AMP Activated Alpha 1 (PRKAa1) ELISA Kit; AMPK; AMPKa1; PKA; ACACA kinase; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase; PRKAa1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Kinase, AMP Activated Alpha 1 (PRKAa1).; No significant cross-reactivity or interference between Protein Kinase, AMP Activated Alpha 1 (PRKAa1) and analogues was observed.
• Sequence Length: 574

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.63ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, AMP Activated Alpha 1 (PRKAa1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, AMP Activated Alpha 1 (PRKAa1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRKAa1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, AMP Activated Alpha 1 (PRKAa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, AMP Activated Alpha 1 (PRKAa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, AMP Activated Alpha 1 (PRKAa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, AMP Activated Alpha 1 (PRKAa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PRKAa1 elisa kit

Enzyme & Kinase; Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 29124503
• NCBI GeneID: 5562
• Molecular Weight: 65,523 Da
• NCBI Official Full Name: Protein kinase, AMP-activated, alpha 1 catalytic subunit
• NCBI Official Synonym Full Names: protein kinase AMP-activated catalytic subunit alpha 1
• NCBI Official Symbol: PRKAA1
• NCBI Official Synonym Symbols: AMPK; AMPKa1
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Gene Name: PRKAA1
• UniProt Synonym Gene Names: AMPK1; HMGCR kinase

NCBI Description

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

AMPKA1: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of a number of metabolic enzymes and transcription regulators. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Two isoforms of the human protein are produced by alternative splicing.

Protein type: AMPK subfamily; Autophagy; CAMK group; CAMKL family; EC 2.7.11.1; EC 2.7.11.26; EC 2.7.11.27; EC 2.7.11.31; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: apical plasma membrane; cytoplasm; cytosol; intracellular; nuclear speck; nucleoplasm; nucleotide-activated protein kinase complex; nucleus

Molecular Function: [acetyl-CoA carboxylase] kinase activity; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity; AMP-activated protein kinase activity; ATP binding; cAMP-dependent protein kinase activity; chromatin binding; histone serine kinase activity; kinase binding; metal ion binding; protein binding; protein C-terminus binding; protein kinase activity; tau-protein kinase activity

Biological Process: activation of MAPK activity; CAMKK-AMPK signaling cascade; cell cycle arrest; cellular response to ethanol; cellular response to glucose starvation; cellular response to hydrogen peroxide; cellular response to hypoxia; cellular response to nutrient levels; cellular response to organonitrogen compound; cellular response to prostaglandin E stimulus; cholesterol biosynthetic process; cold acclimation; fatty acid biosynthetic process; fatty acid homeostasis; fatty acid oxidation; glucose homeostasis; glucose metabolic process; lipid biosynthetic process; macroautophagy; negative regulation of apoptosis; negative regulation of glucose import in response to insulin stimulus; negative regulation of glucosylceramide biosynthetic process; negative regulation of lipid catabolic process; negative regulation of TOR signaling; positive regulation of autophagy; positive regulation of cell proliferation; positive regulation of cholesterol biosynthetic process; positive regulation of gene expression; positive regulation of glycolysis; positive regulation of skeletal muscle tissue development; protein amino acid phosphorylation; protein heterooligomerization; regulation of circadian rhythm; regulation of energy homeostasis; regulation of macroautophagy; regulation of peptidyl-serine phosphorylation; regulation of transcription, DNA-templated; response to activity; response to caffeine; response to camptothecin; response to gamma radiation; response to hypoxia; response to UV; rhythmic process; signal transduction; transcription, DNA-dependent; Wnt signaling pathway