Deiodinase, Iodothyronine, Type II (DIO2) Recombinant Protein | DIO2 recombinant protein

Recombinant Deiodinase, Iodothyronine, Type II (DIO2)

Cat. Num. AAA2097719

• Gene Names: Dio2; 5DII; DIOII; AI324267
• Reactivity: Mouse
• Applications: SDS-Page, Western Blot
• Purity: >95%
• Synonyms: Deiodinase; Iodothyronine; Type II (DIO2); Recombinant Deiodinase; 5DII; D2; SelY; TXDI2; Thyroxine Deiodinase; Type II; Type-II 5'-deiodinase; DIO2 recombinant protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: >95%
• Form/Format: Freeze-dried powder
• Sequence Length: 266

• Applicable Applications for DIO2 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Expression System: Prokaryotic expression
• Tag: N-terminal His Tag
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Fragment: Ser30~Phe259
• Isoelectric Point: 5.8

SDS-Page

Product Categories/Family for DIO2 recombinant protein

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 6753638
• NCBI GeneID: 13371
• NCBI Accession #: NP_034180.1
• NCBI GenBank Nucleotide #: NM_010050.3
• UniProt Accession #: Q9Z1Y9; Q05A70; Q9JHH1
• Molecular Weight: Predicted Molecular Weight: 72.4kDa
• NCBI Official Full Name: type II iodothyronine deiodinase
• NCBI Official Synonym Full Names: deiodinase, iodothyronine, type II
• NCBI Official Symbol: Dio2
• NCBI Official Synonym Symbols: 5DII; DIOII; AI324267
• NCBI Protein Information: type II iodothyronine deiodinase
• UniProt Protein Name: Type II iodothyronine deiodinase
• Protein Family: Type II iodothyronine deiodinase
• UniProt Gene Name: Dio2

NCBI Description

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is highly expressed in brain, placenta and mammary gland. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. Knockout studies in mice suggest that this gene may play an important role in brown adipose tissue lipogenesis, auditory function, and bone formation. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, May 2016]

Uniprot Description

DIO2: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. Belongs to the iodothyronine deiodinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.97.1.10; Membrane protein, integral; Oxidoreductase

Chromosomal Location of Human Ortholog: 12|12 D3

Cellular Component: integral component of membrane; membrane

Molecular Function: oxidoreductase activity; thyroxine 5'-deiodinase activity; ubiquitin protein ligase binding

Biological Process: hormone biosynthetic process; response to cold; thyroid hormone catabolic process; thyroid hormone generation; thyroid hormone metabolic process