Antibody Pairs Support Pack 1 Reagent
Antibody Pairs Support Pack 1
Synonyms
Antibody Pairs Support Pack 1; Antibody Pairs Support Pack 1 reagent
Reagents and Materials
Coating Buffer - 1 bottle of powder
Blocking Buffer - 1 bottle of powder
Standard Diluent - 1 bottle of powder
Detection Antibody Diluent - 1 bottle of powder
Streptavidin-HRP Diluent - 1 bottle of powder
Wash Buffer - 1 bottle of powder
Streptavidin-HRP 1 bottle - 0.6 mL
Substrate Solution 1 bottle - 48 mL each
Stop Solution 1 bottle - 30 mL each
Reagents and Applications
Coating Buffer - Used for coating antibody on the wells of 96-well strip plate. Prepare a working solution by diluting the Coating Buffer with deionized or distilled water before the experiment. 1 bottle of Coating Buffer can be used for preparing 220mL working solution.
Blocking Buffer - Used for blocking of unspecific binding sites of the plate. Prepare a working solution by diluting the Blocking Buffer with deionized or distilled water before the experiment. 1 bottle of Blocking Buffer can be used for preparing 120mL working solution.
Standard Diluent - Used for dilution of Standard. Prepare a working solution by diluting the Standard Diluent reagent with deionized or distilled water before the experiment. 1 bottle of Standard Diluent can be used for preparing 80mL working solution.
Detection Antibody Diluent - Used for dilution of Detection Antibody. Prepare a working solution by diluting the Detection Antibody Diluent reagent with deionized or distilled water before the experiment. 1 bottle of Detection Antibody Diluent can be used for preparing 60mL working solution.
Streptavidin-HRP Diluent - Used for dilution of Streptavidin-HRP. Prepare a working solution by diluting the Streptavidin-HRP Diluent with deionized or distilled water before the experiment. 1 bottle of Streptavidin-HRP Diluent can be used for preparing 60mL working solution.
Wash Buffer - For plate washing. Prepare a working solution by diluting the Wash Buffer with deionized or distilled water before the experiment. 1 bottle of Wash Buffer can be used for preparing 2000mL working solution.
Streptavidin-HRP - Steptavidin-HRP can specifically bind biotinylated antibodies. Prepare a working solution by diluting the Streptavidin-HRP with Streptavidin-HRP Diluent working solution. The dilution ratio was 1:100. 1 bottle can be used for preparing 60mL working solution.
Substrate Solution - Used for chromogenic substrate.
Stop Solution - Used for stopping chromogenic reaction.
Blocking Buffer - Used for blocking of unspecific binding sites of the plate. Prepare a working solution by diluting the Blocking Buffer with deionized or distilled water before the experiment. 1 bottle of Blocking Buffer can be used for preparing 120mL working solution.
Standard Diluent - Used for dilution of Standard. Prepare a working solution by diluting the Standard Diluent reagent with deionized or distilled water before the experiment. 1 bottle of Standard Diluent can be used for preparing 80mL working solution.
Detection Antibody Diluent - Used for dilution of Detection Antibody. Prepare a working solution by diluting the Detection Antibody Diluent reagent with deionized or distilled water before the experiment. 1 bottle of Detection Antibody Diluent can be used for preparing 60mL working solution.
Streptavidin-HRP Diluent - Used for dilution of Streptavidin-HRP. Prepare a working solution by diluting the Streptavidin-HRP Diluent with deionized or distilled water before the experiment. 1 bottle of Streptavidin-HRP Diluent can be used for preparing 60mL working solution.
Wash Buffer - For plate washing. Prepare a working solution by diluting the Wash Buffer with deionized or distilled water before the experiment. 1 bottle of Wash Buffer can be used for preparing 2000mL working solution.
Streptavidin-HRP - Steptavidin-HRP can specifically bind biotinylated antibodies. Prepare a working solution by diluting the Streptavidin-HRP with Streptavidin-HRP Diluent working solution. The dilution ratio was 1:100. 1 bottle can be used for preparing 60mL working solution.
Substrate Solution - Used for chromogenic substrate.
Stop Solution - Used for stopping chromogenic reaction.
Important Notes
Appropriate care should be exercised when using these reagents, including gloves, glasses, lab coats, and good laboratory procedures.
Preparation and Storage
Streptavidin-HRP should be stored at -20 degree C, others are valid for 6 months at 4 degree C. Store diluted (reconstituted powder) or working solutions at 4 degree C for up to one week for use in subsequent experiments.
Related Product Information for Antibody Pairs Support Pack 1 reagent
Antibody Pairs Support Pack 1 is intended to use together with Antibody Pairs for ELISA/CLIA (sandwich method) kit preparation. This pack provides sufficient reagents for preparation of 5 sandwich ELISA/CLIA plates. All reagents should be used at room temperature and the pack is intended for research purposes only.
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Product Notes
The Antibody Pairs Support Pack 1 (Catalog #AAA2090685) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Antibody Pairs Support Pack 1, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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