Mouse Transferrin (TRF) DIY ELISA Kit | TRF diy elisa kit

Mouse Transferrin (TRF) ELISA Kit DIY Materials

Cat. Num. AAA2089239

• Gene Names: TF; TFQTL1; PRO1557; PRO2086; HEL-S-71p
• Reactivity: Mouse
• Synonyms: Transferrin (TRF); Mouse Transferrin (TRF) ELISA Kit DIY Materials; TF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin; TRF diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Transferrin (TRF). No significant cross-reactivity or interference between Transferrin (TRF) and analogues was observed.
• Sequence Length: 698

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 12.35-1000ng/mL
• Sensitivity: 5.78ng/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for TRF diy elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 339453
• NCBI GeneID: 7018
• Molecular Weight: 77,064 Da
• NCBI Official Full Name: transferrin
• NCBI Official Synonym Full Names: transferrin
• NCBI Official Symbol: TF
• NCBI Official Synonym Symbols: TFQTL1; PRO1557; PRO2086; HEL-S-71p
• NCBI Protein Information: serotransferrin
• UniProt Protein Name: Serotransferrin
• Protein Family: TBP-related factor
• UniProt Gene Name: TF
• UniProt Synonym Gene Names: Transferrin

NCBI Description

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

Uniprot Description

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: apical plasma membrane; basal plasma membrane; cell surface; coated pit; cytoplasmic vesicle; early endosome; endocytic vesicle; endosome membrane; extracellular region; extracellular space; extrinsic to external side of plasma membrane; late endosome; perinuclear region of cytoplasm; recycling endosome; vesicle

Molecular Function: ferrous iron binding; protein binding

Biological Process: cellular iron ion homeostasis; iron ion homeostasis; platelet degranulation; positive regulation of receptor-mediated endocytosis; regulation of protein stability; retinal homeostasis; transferrin transport

Disease: Atransferrinemia