Rat Apolipoprotein B (APOB) DIY ELISA Kit | APOB diy elisa kit

Rat Apolipoprotein B (APOB) ELISA Kit DIY Materials

Cat. Num. AAA2089227

• Gene Names: APOB; FLDB; LDLCQ4; apoB-48; apoB-100
• Reactivity: Rat
• Synonyms: Apolipoprotein B (APOB); Rat Apolipoprotein B (APOB) ELISA Kit DIY Materials; Apo-B; FLDB; Apo B-100; Apo B-48; APOB diy elisa kit

• Reactivity: Rat
• Specificity: The Antibody Pairs in the kit have high sensitivity and excellent specificity for detection of APOB.
• Sequence Length: 1670

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Sandwich
• Detection Range: 31.2-2,000ng/mL
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOB diy elisa kit

Intended Uses: This 'Do it Yourself (DIY)' assay kit contains materials for developing your own immunoassays. This may include the development of sandwich ELISA to measure APOB in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids in vitro. This kit contains sufficient materials for preparation of at least ten 96-well plates. Sample Preparation, Assay Protocol and Detection Range for Reference are provided as suggestions only. Researchers should optimize the use of kit materials and protocols for their own model system and determine if the kit is suitable for their samples and immunoassay.

Product Categories/Family for APOB diy elisa kit

Cardiovascular biology

NCBI and Uniprot Product Information

• NCBI GI #: 553189
• NCBI GeneID: 338
• Molecular Weight: 515,605 Da
• NCBI Official Full Name: apolipoprotein B, partial
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: APOB
• NCBI Official Synonym Symbols: FLDB; LDLCQ4; apoB-48; apoB-100
• NCBI Protein Information: apolipoprotein B-100
• UniProt Protein Name: Apolipoprotein B-100
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOB
• UniProt Synonym Gene Names: Apo B-100; Apo B-48

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Uniprot Description

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Carrier; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: actin cytoskeleton; cell soma; chylomicron; cytoplasm; cytosol; early endosome; endoplasmic reticulum lumen; endoplasmic reticulum membrane; endosome membrane; extracellular region; extracellular space; Golgi apparatus; intracellular membrane-bound organelle; plasma membrane

Molecular Function: cholesterol transporter activity; heparin binding; low-density lipoprotein receptor binding; phospholipid binding; protein binding

Biological Process: cellular protein catabolic process; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; leukocyte migration; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein metabolic process; receptor-mediated endocytosis; response to virus; retinoid metabolic process

Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1