Bovine Alpha-Lactalbumin (aLA) DIY ELISA Kit | aLA diy elisa kit

Bovine Alpha-Lactalbumin (aLA) ELISA Kit DIY Materials

Cat. Num. AAA2088975

• Gene Names: LALBA; LYZG
• Reactivity: Bovine
• Synonyms: Alpha-Lactalbumin (aLA); Bovine Alpha-Lactalbumin (aLA) ELISA Kit DIY Materials; LALBA; LYZL7; Lactose Synthase B Protein; Lysozyme Like Protein 7; aLA diy elisa kit

• Reactivity: Bovine
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-Lactalbumin (aLA). No significant cross-reactivity or interference between Alpha-Lactalbumin (aLA) and analogues was observed.
• Sequence Length: 142

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Milk and other biological fluids.
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.285ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for aLA diy elisa kit

Tumor immunity; Infection immunity; Endocrinology; Reproductive science; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4504947
• NCBI GeneID: 3906
• NCBI Accession #: NP_002280.1
• NCBI GenBank Nucleotide #: NM_002289.2
• UniProt Accession #: P00709; Q6FGX0; Q9UDK4
• Molecular Weight: 16,225 Da
• NCBI Official Full Name: alpha-lactalbumin
• NCBI Official Synonym Full Names: lactalbumin alpha
• NCBI Official Symbol: LALBA
• NCBI Official Synonym Symbols: LYZG
• NCBI Protein Information: alpha-lactalbumin
• UniProt Protein Name: Alpha-lactalbumin
• UniProt Gene Name: LALBA
• UniProt Synonym Gene Names: LYZL7

NCBI Description

This gene encodes alpha-lactalbumin, a principal protein of milk. Alpha-lactalbumin forms the regulatory subunit of the lactose synthase (LS) heterodimer and beta 1,4-galactosyltransferase (beta4Gal-T1) forms the catalytic component. Together, these proteins enable LS to produce lactose by transfering galactose moieties to glucose. As a monomer, alpha-lactalbumin strongly binds calcium and zinc ions and may possess bactericidal or antitumor activity. A folding variant of alpha-lactalbumin, called HAMLET, likely induces apoptosis in tumor and immature cells. [provided by RefSeq, Jul 2008]

Uniprot Description

LALBA: Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins. Belongs to the glycosyl hydrolase 22 family.

Protein type: Apoptosis; Secreted; Secreted, signal peptide; Transferase

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: extracellular space; Golgi lumen; Golgi membrane

Molecular Function: lysozyme activity

Biological Process: apoptosis; cell wall catabolic process; cell-cell signaling; defense response to bacterium; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; lactose biosynthetic process; signal transduction