Mouse Peroxisome Proliferator Activated Receptor Gamma (PPARg) DIY ELISA Kit | PPARg diy elisa kit

Mouse Peroxisome Proliferator Activated Receptor Gamma (PPARg) ELISA Kit DIY Materials

Cat. Num. AAA2088909

• Reactivity: Mouse
• Synonyms: Peroxisome Proliferator Activated Receptor Gamma (PPARg); Mouse Peroxisome Proliferator Activated Receptor Gamma (PPARg) ELISA Kit DIY Materials; PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Gamma (PPARg). No significant cross-reactivity or interference betweenPeroxisome Proliferator Activated Receptor Gamma (PPARg) and analogues was observed.
• Sequence Length: 477

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.065ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for PPARg diy elisa kit

Metabolic pathway; Endocrinology; Cardiovascular biology; Developmental science; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 17978516
• Molecular Weight: 21,580 Da
• NCBI Official Full Name: peroxisome proliferator activated receptor gamma
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma
• Protein Family: Peroxisome proliferator-activated receptor
• UniProt Gene Name: PPARG
• UniProt Synonym Gene Names: NR1C3; PPAR-gamma

Uniprot Description

PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 3p25.2

Cellular Component: cytosol; Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function: alpha-actinin binding; arachidonic acid binding; chromatin binding; DNA binding; drug binding; enzyme binding; identical protein binding; ligand-dependent nuclear receptor activity; ligand-dependent nuclear receptor transcription coactivator activity; prostaglandin receptor activity; protein binding; retinoid X receptor binding; sequence-specific DNA binding; transcription activator binding; transcription factor activity; transcription factor binding

Biological Process: caspase activation; cell fate commitment; cell maturation; cellular response to insulin stimulus; epithelial cell differentiation; glucose homeostasis; innate immune response; lipid homeostasis; lipid metabolic process; lipoprotein transport; long-chain fatty acid transport; low-density lipoprotein receptor biosynthetic process; monocyte differentiation; negative regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; placenta development; positive regulation of fat cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of blood pressure; regulation of circadian rhythm; response to lipid; response to low density lipoprotein stimulus; response to nutrient; response to retinoic acid; signal transduction; transcription initiation from RNA polymerase II promoter; white fat cell differentiation

Disease: Carotid Intimal Medial Thickness 1; Diabetes Mellitus, Noninsulin-dependent; Lipodystrophy, Familial Partial, Type 3; Obesity