Human Alpha-Hemoglobin Stabilizing Protein (aHSP) DIY ELISA Kit | aHSP diy elisa kit
Human Alpha-Hemoglobin Stabilizing Protein (aHSP) ELISA Kit DIY Materials
Gene Names
AHSP; EDRF; ERAF
Reactivity
Human
Synonyms
Alpha-Hemoglobin Stabilizing Protein (aHSP); Human Alpha-Hemoglobin Stabilizing Protein (aHSP) ELISA Kit DIY Materials; A-HSP; ERAF; EDRF; Erythroid Associated Factor; Erythroid differentiation-related factor; aHSP diy elisa kit
Reactivity
Human
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Alpha-Hemoglobin Stabilizing Protein (aHSP). No significant cross-reactivity or interference between Alpha-Hemoglobin Stabilizing Protein (aHSP) and analogues was observed.
Sequence Length
102
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
31.2-2,000pg/mL
Sensitivity
13.3pg/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for aHSP diy elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,840 Da
NCBI Official Full Name
alpha-hemoglobin-stabilizing protein
NCBI Official Synonym Full Names
alpha hemoglobin stabilizing protein
NCBI Official Symbol
AHSP
NCBI Official Synonym Symbols
EDRF; ERAF
NCBI Protein Information
alpha-hemoglobin-stabilizing protein
UniProt Protein Name
Alpha-hemoglobin-stabilizing protein
Protein Family
UniProt Gene Name
AHSP
UniProt Synonym Gene Names
EDRF; ERAF
NCBI Description
This gene encodes a molecular chaperone which binds specifically to free alpha-globin and is involved in hemoglobin assembly. The encoded protein binds to monomeric alpha-globin until it has been transferred to beta-globin to form a heterodimer, which in turn binds to another heterodimer to form the stable tetrameric hemoglobin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Uniprot Description
AHSP: Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. Belongs to the AHSP family.
Chromosomal Location of Human Ortholog: 16p11.2
Molecular Function: protein binding
Research Articles on aHSP
Similar Products
Product Notes
The Human aHSP ahsp (Catalog #AAA2088548) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088548 DIY ELISA Kit recognizes Human aHSP. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the aHSP ahsp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-Hemoglobin Stabilizing Protein (aHSP), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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