Glutathione (GSH) DIY ELISA Kit | GSH diy elisa kit

General Glutathione (GSH) ELISA Kit DIY Materials

Cat. Num. AAA2088507

• Gene Names: GSTM2; GST4; GSTM; GTHMUS; GSTM2-2
• Reactivity: Pan-species (General)
• Synonyms: Glutathione (GSH); General Glutathione (GSH) ELISA Kit DIY Materials; L-Reduced Glutathione; GSH diy elisa kit

• Reactivity: Pan-species (General)
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Glutathione (GSH). No significant cross-reactivity or interference between Glutathione (GSH) and analogues was observed.
• Sequence Length: 191

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 1.23-100ug/mL
• Sensitivity: 0.48ug/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for GSH diy elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 215277000
• NCBI GeneID: 2946
• NCBI Accession #: NP_001135840.1
• NCBI GenBank Nucleotide #: NM_001142368.1
• UniProt Accession #: P28161; Q2M318; Q5TZY5; Q8WWE1; B4DRY4; E9PEM9
• Molecular Weight: 22,644 Da
• NCBI Official Full Name: glutathione S-transferase Mu 2 isoform 2
• NCBI Official Synonym Full Names: glutathione S-transferase mu 2
• NCBI Official Symbol: GSTM2
• NCBI Official Synonym Symbols: GST4; GSTM; GTHMUS; GSTM2-2
• NCBI Protein Information: glutathione S-transferase Mu 2
• UniProt Protein Name: Glutathione S-transferase Mu 2
• UniProt Gene Name: GSTM2
• UniProt Synonym Gene Names: GST4

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTM2: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family.

Protein type: EC 2.5.1.18; Motility/polarity/chemotaxis; Transferase

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytoplasm; cytosol; sarcoplasmic reticulum

Molecular Function: enzyme binding; glutathione binding; glutathione peroxidase activity; glutathione transferase activity; protein binding; protein homodimerization activity; receptor binding

Biological Process: glutathione metabolic process; linoleic acid metabolic process; nitrobenzene metabolic process; regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; xenobiotic catabolic process