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Mouse Histone H3 (H3) DIY ELISA Kit | H3 diy elisa kit

Mouse Histone H3 (H3) ELISA Kit DIY Materials

Reactivity
Mouse
Synonyms
Histone H3 (H3); Mouse Histone H3 (H3) ELISA Kit DIY Materials; H3 diy elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Histone H3 (H3). No significant cross-reactivity or interference between Histone H3 (H3) and analogues was observed.
Sequence Length
136
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.115ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for H3 diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
15,406 Da
NCBI Official Full Name
histone H3
UniProt Protein Name
Histone H3.3
Protein Family
UniProt Gene Name
H3

Uniprot Description

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

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Product Notes

The Mouse H3 h3 (Catalog #AAA2088505) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088505 DIY ELISA Kit recognizes Mouse H3. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the H3 h3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3 (H3), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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