Human Nesfatin 1 (NES1) DIY ELISA Kit | NES1 diy elisa kit
Human Nesfatin 1 (NES1) ELISA Kit DIY Materials
Gene Names
NUCB2; NEFA; HEL-S-109
Reactivity
Human
Synonyms
Nesfatin 1 (NES1); Human Nesfatin 1 (NES1) ELISA Kit DIY Materials; NES1 diy elisa kit
Reactivity
Human
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Nesfatin 1 (NES1). No significant cross-reactivity or interference between Nesfatin 1 (NES1) and analogues was observed.
Sequence Length
420
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Competitive Inhibition ELISA for Antigen Detection
Samples
Serum, plasma and other biological fluids.
Detection Range
617.3-50000pg/mL
Sensitivity
251.4pg/mL
Reagent Contents
Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,201 Da
NCBI Official Full Name
nucleobindin-2 isoform 1
NCBI Official Synonym Full Names
nucleobindin 2
NCBI Official Symbol
NUCB2
NCBI Official Synonym Symbols
NEFA; HEL-S-109
NCBI Protein Information
nucleobindin-2
UniProt Protein Name
Nucleobindin-2
UniProt Gene Name
NUCB2
UniProt Synonym Gene Names
NEFA
NCBI Description
This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]
Uniprot Description
NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Vesicle
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: cytosol; ER-Golgi intermediate compartment; extracellular space; plasma membrane
Molecular Function: protein binding
Research Articles on NES1
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Product Notes
The Human NES1 nucb2 (Catalog #AAA2088490) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088490 DIY ELISA Kit recognizes Human NES1. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the NES1 nucb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nesfatin 1 (NES1), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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