Porcine Insulin Like Growth Factor Binding Protein 3 (IGFBP3) DIY ELISA Kit | IGFBP3 diy elisa kit
Porcine Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit DIY Materials
Gene Names
IGFBP3; IGFBP-3
Reactivity
Porcine
Synonyms
Insulin Like Growth Factor Binding Protein 3 (IGFBP3); Porcine Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit DIY Materials; BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex; IGFBP3 diy elisa kit
Reactivity
Porcine
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 3 (IGFBP3). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 3 (IGFBP3) and analogues was observed.
Sequence Length
291
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.138ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for IGFBP3 diy elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,570 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 3
NCBI Official Symbol
IGFBP3
NCBI Official Synonym Symbols
IGFBP-3
NCBI Protein Information
insulin-like growth factor-binding protein 3
UniProt Protein Name
Insulin-like growth factor-binding protein 3
Protein Family
UniProt Gene Name
IGFBP3
UniProt Synonym Gene Names
IBP-3; IGF-binding protein 3; IGFBP-3
Uniprot Description
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.
Research Articles on IGFBP3
Similar Products
Product Notes
The Porcine IGFBP3 igfbp3 (Catalog #AAA2088340) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088340 DIY ELISA Kit recognizes Porcine IGFBP3. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the IGFBP3 igfbp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin Like Growth Factor Binding Protein 3 (IGFBP3), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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