Human Interleukin 1 Receptor Associated Kinase 2 (IRAK2) ELISA Kit | IRAK2 elisa kit

Human Interleukin 1 Receptor Associated Kinase 2 (IRAK2) ELISA Kit

Cat. Num. AAA2088158

• Gene Names: IRAK2; IRAK-2
• Reactivity: Human
• Synonyms: Interleukin 1 Receptor Associated Kinase 2 (IRAK2); Human Interleukin 1 Receptor Associated Kinase 2 (IRAK2) ELISA Kit; IRAK2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Associated Kinase 2 (IRAK2).; No significant cross-reactivity or interference between Interleukin 1 Receptor Associated Kinase 2 (IRAK2) and analogues was observed.
• Sequence Length: 625

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.27ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 2 (IRAK2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 2 (IRAK2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRAK2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 2 (IRAK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 2 (IRAK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 2 (IRAK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Associated Kinase 2 (IRAK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IRAK2 elisa kit

Signal transduction; Enzyme & Kinase; Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 58530886
• NCBI GeneID: 3656
• NCBI Accession #: NP_001561.3
• NCBI GenBank Nucleotide #: NM_001570.3
• UniProt Accession #: O43187; Q08AG6; Q5K546; B4DQZ6
• Molecular Weight: 69,433 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase-like 2
• NCBI Official Synonym Full Names: interleukin 1 receptor associated kinase 2
• NCBI Official Symbol: IRAK2
• NCBI Official Synonym Symbols: IRAK-2
• NCBI Protein Information: interleukin-1 receptor-associated kinase-like 2
• UniProt Protein Name: Interleukin-1 receptor-associated kinase-like 2
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK2
• UniProt Synonym Gene Names: IRAK-2

NCBI Description

IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAK2: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Interacts with MYD88. IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Protein type: IRAK family; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytoplasm; cytosol; endosome membrane; nucleus; plasma membrane

Molecular Function: protein binding; protein heterodimerization activity; protein homodimerization activity; protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; inhibition of NF-kappaB transcription factor; JNK cascade; MyD88-dependent toll-like receptor signaling pathway; protein amino acid phosphorylation; regulation of cytokine and chemokine mediated signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway