Interleukin 1 Beta (IL1b) Protein | IL1b protein

Eukaryotic Interleukin 1 Beta (IL1b)

Cat. Num. AAA2086717

• Gene Names: Il1b; Il-1b; IL-1beta
• Applications: SDS-Page, Western Blot
• Purity: > 97%
• Synonyms: Interleukin 1 Beta (IL1b); Eukaryotic Interleukin 1 Beta (IL1b); IL1-B; IL1-Beta; IL1F2; IL-1beta; Interleukin-1 Family Member 2; Catabolin; IL1b protein

• Host: 293F cell
• Purity/Purification: > 97%
• Form/Format: Freeze-dried powder
• Sequence Length: 269

• Applicable Applications for IL1b protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Val118~Ser269
• Tag: N-terminal His Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Eukaryotic expression
• Organism Species: Mouse
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 8
• Subcellular Location: Secreted, Lysosome, Cytoplasm, Exosome
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Testing Data

Product Categories/Family for IL1b protein

Eukaryotic proteins

NCBI and Uniprot Product Information

• NCBI GI #: 6680415
• NCBI GeneID: 16176
• NCBI Accession #: NP_032387.1
• NCBI GenBank Nucleotide #: NM_008361.4
• UniProt Accession #: P10749; Q2M4J6
• Molecular Weight: 19.0kDa; 19kDa
• NCBI Official Full Name: interleukin-1 beta
• NCBI Official Synonym Full Names: interleukin 1 beta
• NCBI Official Symbol: Il1b
• NCBI Official Synonym Symbols: Il-1b; IL-1beta
• NCBI Protein Information: interleukin-1 beta
• UniProt Protein Name: Interleukin-1 beta
• Protein Family: Interleukin
• UniProt Gene Name: Il1b
• UniProt Synonym Gene Names: IL-1 beta

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. [provided by RefSeq, Aug 2015]

Uniprot Description

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2 F1|2 62.97 cM

Cellular Component: extracellular region; extracellular space; secretory granule; vesicle

Molecular Function: cytokine activity; interleukin-1 receptor binding; protein domain specific binding

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; elevation of cytosolic calcium ion concentration; germ cell programmed cell death; hyaluronan biosynthetic process; inflammatory response; interleukin-1 beta production; leukocyte migration; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; memory; negative regulation of cell proliferation; negative regulation of glutamate secretion; negative regulation of insulin receptor signaling pathway; negative regulation of lipid catabolic process; negative regulation of lipid metabolic process; negative regulation of MAP kinase activity; negative regulation of neurogenesis; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; neutrophil chemotaxis; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of astrocyte differentiation; positive regulation of chemokine biosynthetic process; positive regulation of fever; positive regulation of glial cell differentiation; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of immature T cell proliferation in the thymus; positive regulation of interferon-gamma production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-6 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of interleukin-8 production; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of lipid catabolic process; positive regulation of membrane protein ectodomain proteolysis; positive regulation of mitosis; positive regulation of neuron apoptosis; positive regulation of NF-kappaB import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of phagocytosis; positive regulation of prostaglandin secretion; positive regulation of protein amino acid phosphorylation; positive regulation of stress-activated MAPK cascade; positive regulation of T cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein kinase B signaling cascade; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of insulin secretion; regulation of nitric-oxide synthase activity; response to ATP; response to carbohydrate stimulus; response to lipopolysaccharide; sequestering of triacylglycerol; social behavior