IL12 active protein

IL12 23-335aa (IL12b)/ 23-215aa (IL12a), Mouse, His-tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206788

• Gene Names: Il12b; p40; Il-12b; Il12p40; Il-12p40
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: IL12; IL12 23-335aa (IL12b)/ 23-215aa (IL12a); Mouse; His-tag; Baculovirus (Bioactivity Validated); Interleukin 12 (subunit beta/alpha); IL12b/IL12a; Il-12b/Il-12a; IL-12p40/Il-12p35; Il12p40/Il12p35; p40/p35; IL12 active protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: IL12B (p40) MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS IL12A (p35) RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSAHHHHHH
• Sequence Length: 335

• Applicable Applications for IL12 active protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Biological Activity: The activity is determined by the IFN-g ELISA in a using NK-92 cell. The ED50 for this effect is less or equal to 10 ng/ml.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for IL12 active protein

IL12b/IL12a, also known as interleukin 12 (subunit beta/alpha), is a cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. It is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. As it is associated with IL23A to form the IL-23 interleukin, it induces autoimmune inflammation and is responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Recombinant mouse IL12b/IL12a protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for IL12 active protein

Immunology; Hormones & Cytokines

References

Zhang X., et al, (2015) J Immunol. 195:1665-1675.; Zamani A., et al, (2017) Immune Netw. 17:186-191.

NCBI and Uniprot Product Information

• NCBI GI #: 735997434
• NCBI GeneID: 16160
• NCBI Accession #: NP_001290173; NP_032377
• NCBI GenBank Nucleotide #: NM_001303244.1
• UniProt Accession #: P43432; Q9QUM1
• Molecular Weight: 35.7kDa (p40) & 22.5kDa (p35) (313aa / 199aa) The heterodimeric Il12 has a predicted molecular weight of 58.3kDa (512aa). Molecular weight on SDS-PAGE will appear higher
• NCBI Official Full Name: interleukin-12 subunit beta
• NCBI Official Synonym Full Names: interleukin 12b
• NCBI Official Symbol: Il12b
• NCBI Official Synonym Symbols: p40; Il-12b; Il12p40; Il-12p40
• NCBI Protein Information: interleukin-12 subunit beta
• UniProt Protein Name: Interleukin-12 subunit beta
• UniProt Gene Name: Il12b
• UniProt Synonym Gene Names: IL-12B; CLMF p40

NCBI Description

This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23. [provided by RefSeq, Dec 2014]

Uniprot Description

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.