TFPI recombinant protein

TFPI, 29-304aa, Human, His-tag, Baculovirus

Cat. Num. AAA206659

• Gene Names: TFPI; EPI; TFI; LACI; TFPI1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TFPI; 29-304aa; Human; His-tag; Baculovirus; Tissue factor pathway inhibitor isoform; EPI; LACI; TFI; TFPI1; TFPI recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPDSEEDEE HTIITDTELP PLKLMHSFCA FKADDGPCKA IMKRFFFNIF TRQCEEFIYG GCEGNQNRFE SLEECKKMCT RDNANRIIKT TLQQEKPDFC FLEEDPGICR GYITRYFYNN QTKQCERFKY GGCLGNMNNF ETLEECKNIC EDGPNGFQVD NYGTQLNAVN NSLTPQSTKV PSLFEFHGPS WCLTPADRGL CRANENRFYY NSVIGKCRPF KYSGCGGNEN NFTSKQECLR ACKKGFIQRI SKGGLIKTKR KRKKQRVKIA YEEIFVKNMH HHHHH
• Sequence Length: 304

• Applicable Applications for TFPI recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TFPI recombinant protein

TFPI, also known as tissue factor pathway inhibitor isoform, is the major regulator of tissue factor (TF)-induced coagulation. It is predominantly produced by microvascular endothelial cells, though it is also found in platelets, monocytes, smooth muscle cells, and plasma. It is an anticoagulant protein that inhibits early phases of the procoagulant response. It has been shown to be associated with breast cancer pathogenesis. It consists of a highly negatively charged amino-terminus, three tandemly linked Kunitz domains, and a highly positively charged carboxy-terminus. It exhibits significant homology with human inter-alpha-trypsin inhibitor and bovine basic pancreatic trypsin inhibitor. Recombinant human TFPI, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for TFPI recombinant protein

Cardiovascular

References

Maroney SA., et al. (2010) Thromb Res. 1:S52-S56.; Ali HO., et al. (2016) PLoS One. 11:e0152114.

NCBI and Uniprot Product Information

• NCBI GI #: 5454114
• NCBI GeneID: 7035
• NCBI Accession #: NP_006278
• NCBI GenBank Nucleotide #: NM_006287.5
• UniProt Accession #: P10646; O95103; Q53TS4
• Molecular Weight: 33kDa (285aa) 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: tissue factor pathway inhibitor isoform a
• NCBI Official Synonym Full Names: tissue factor pathway inhibitor
• NCBI Official Symbol: TFPI
• NCBI Official Synonym Symbols: EPI; TFI; LACI; TFPI1
• NCBI Protein Information: tissue factor pathway inhibitor
• UniProt Protein Name: Tissue factor pathway inhibitor
• Protein Family: Tissue factor pathway inhibitor
• UniProt Gene Name: TFPI
• UniProt Synonym Gene Names: LACI; TFPI1; TFPI; EPI; LACI

NCBI Description

This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization. [provided by RefSeq, Jul 2016]

Uniprot Description

Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.